Entamoeba and Giardia parasites implicated as hosts of CRESS viruses

Cormac M. Kinsella, Aldert Bart, Martin Deijs, Patricia Broekhuizen, Joanna Kaczorowska, Maarten F. Jebbink, Tom van Gool, Matthew Cotten, Lia van der Hoek

Research output: Contribution to JournalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Metagenomic techniques have enabled genome sequencing of unknown viruses without isolation in cell culture, but information on the virus host is often lacking, preventing viral characterisation. High-throughput methods capable of identifying virus hosts based on genomic data alone would aid evaluation of their medical or biological relevance. Here, we address this by linking metagenomic discovery of three virus families in human stool samples with determination of probable hosts. Recombination between viruses provides evidence of a shared host, in which genetic exchange occurs. We utilise networks of viral recombination to delimit virus-host clusters, which are then anchored to specific hosts using (1) statistical association to a host organism in clinical samples, (2) endogenous viral elements in host genomes, and (3) evidence of host small RNA responses to these elements. This analysis suggests two CRESS virus families (Naryaviridae and Nenyaviridae) infect Entamoeba parasites, while a third (Vilyaviridae) infects Giardia duodenalis. The trio supplements five CRESS virus families already known to infect eukaryotes, extending the CRESS virus host range to protozoa. Phylogenetic analysis implies CRESS viruses infecting multicellular life have evolved independently on at least three occasions.
Original languageEnglish
Article number4620
JournalNature communications
Volume11
Issue number1
DOIs
Publication statusPublished - 1 Dec 2020

Cite this