TY - JOUR
T1 - Establishing reference intervals for triglyceride-containing lipoprotein subfraction metabolites measured using nuclear magnetic resonance spectroscopy in a UK population
AU - Joshi, Roshni
AU - Wannamethee, Goya
AU - Engmann, Jorgen
AU - Gaunt, Tom
AU - Lawlor, Deborah A.
AU - Price, Jackie
AU - Papacosta, Olia
AU - Shah, Tina
AU - Tillin, Therese
AU - Whincup, Peter
AU - Chaturvedi, Nishi
AU - Kivimaki, Mika
AU - Kuh, Diana
AU - Kumari, Meena
AU - Hughes, Alun D.
AU - Casas, Juan P.
AU - Humphries, Steve E.
AU - Hingorani, Aroon D.
AU - on behalf of the UCLEB Consortium
AU - Schmidt, A. Floriaan
PY - 2021/1/1
Y1 - 2021/1/1
N2 - BACKGROUND: Nuclear magnetic resonance (NMR) spectroscopy allows triglycerides to be subclassified into 14 different classes based on particle size and lipid content. We recently showed that these subfractions have differential associations with cardiovascular disease events. Here we report the distributions and define reference interval ranges for 14 triglyceride-containing lipoprotein subfraction metabolites.METHODS: Lipoprotein subfractions using the Nightingale NMR platform were measured in 9073 participants from four cohort studies contributing to the UCL-Edinburgh-Bristol consortium. The distribution of each metabolite was assessed, and reference interval ranges were calculated for a disease-free population, by sex and age group (<55, 55-65, >65 years), and in a subgroup population of participants with cardiovascular disease or type 2 diabetes. We also determined the distribution across body mass index and smoking status.RESULTS: The largest reference interval range was observed in the medium very-low density lipoprotein subclass (2.5th 97.5th percentile; 0.08 to 0.68 mmol/L). The reference intervals were comparable among male and female participants, with the exception of triglyceride in high-density lipoprotein. Triglyceride subfraction concentrations in very-low density lipoprotein, intermediate-density lipoprotein, low-density lipoprotein and high-density lipoprotein subclasses increased with increasing age and increasing body mass index. Triglyceride subfraction concentrations were significantly higher in ever smokers compared to never smokers, among those with clinical chemistry measured total triglyceride greater than 1.7 mmol/L, and in those with cardiovascular disease, and type 2 diabetes as compared to disease-free subjects.CONCLUSION: This is the first study to establish reference interval ranges for 14 triglyceride-containing lipoprotein subfractions in samples from the general population measured using the nuclear magnetic resonance platform. The utility of nuclear magnetic resonance lipid measures may lead to greater insights for the role of triglyceride in cardiovascular disease, emphasizing the importance of appropriate reference interval ranges for future clinical decision making.
AB - BACKGROUND: Nuclear magnetic resonance (NMR) spectroscopy allows triglycerides to be subclassified into 14 different classes based on particle size and lipid content. We recently showed that these subfractions have differential associations with cardiovascular disease events. Here we report the distributions and define reference interval ranges for 14 triglyceride-containing lipoprotein subfraction metabolites.METHODS: Lipoprotein subfractions using the Nightingale NMR platform were measured in 9073 participants from four cohort studies contributing to the UCL-Edinburgh-Bristol consortium. The distribution of each metabolite was assessed, and reference interval ranges were calculated for a disease-free population, by sex and age group (<55, 55-65, >65 years), and in a subgroup population of participants with cardiovascular disease or type 2 diabetes. We also determined the distribution across body mass index and smoking status.RESULTS: The largest reference interval range was observed in the medium very-low density lipoprotein subclass (2.5th 97.5th percentile; 0.08 to 0.68 mmol/L). The reference intervals were comparable among male and female participants, with the exception of triglyceride in high-density lipoprotein. Triglyceride subfraction concentrations in very-low density lipoprotein, intermediate-density lipoprotein, low-density lipoprotein and high-density lipoprotein subclasses increased with increasing age and increasing body mass index. Triglyceride subfraction concentrations were significantly higher in ever smokers compared to never smokers, among those with clinical chemistry measured total triglyceride greater than 1.7 mmol/L, and in those with cardiovascular disease, and type 2 diabetes as compared to disease-free subjects.CONCLUSION: This is the first study to establish reference interval ranges for 14 triglyceride-containing lipoprotein subfractions in samples from the general population measured using the nuclear magnetic resonance platform. The utility of nuclear magnetic resonance lipid measures may lead to greater insights for the role of triglyceride in cardiovascular disease, emphasizing the importance of appropriate reference interval ranges for future clinical decision making.
KW - Aged
KW - Cardiovascular Diseases/blood
KW - Diabetes Mellitus, Type 2/blood
KW - Female
KW - Humans
KW - Lipoproteins/blood
KW - Male
KW - Middle Aged
KW - Nuclear Magnetic Resonance, Biomolecular
KW - Reference Standards
KW - Triglycerides/blood
KW - United Kingdom
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85093973690&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/32936666
U2 - https://doi.org/10.1177/0004563220961753
DO - https://doi.org/10.1177/0004563220961753
M3 - Article
C2 - 32936666
SN - 0004-5632
VL - 58
SP - 47
EP - 53
JO - Annals of clinical biochemistry
JF - Annals of clinical biochemistry
IS - 1
ER -