ESTRADIOL-INDUCED SYNTHESIS OF VITELLOGENIN .3. ISOLATION AND CHARACTERIZATION OF VITELLOGENIN MESSENGER-RNA FROM AVIAN LIVER

G. AB; W. G. Roskam; J. Dijkstra; J. Mulder; M. Willems; A. van der Ende; M. Gruber

doi:https://doi.org/10.1016/0005-2787(76)90355-5

ESTRADIOL-INDUCED SYNTHESIS OF VITELLOGENIN .3. ISOLATION AND CHARACTERIZATION OF VITELLOGENIN MESSENGER-RNA FROM AVIAN LIVER

G. AB, W. G. Roskam, J. Dijkstra, J. Mulder, M. Willems, A. van der Ende, M. Gruber

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Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The messenger RNA of the hormone-induced protein vitellogenin was isolated from the liver of estrogen-treated roosters. Starting from total polysomal RNA, the vitellogenin messenger was purified 67-fold by oligo (dT)-cellulose chromatography and sizing on a sucrose gradient. The messenger was translated in vitro into a 170 000 dalton polypeptide chain, having the immunochemical characteristics of vitellogenin. From electrophoretic and immunochemical analysis of the in vitro product of translation at least 63% of the messenger activity of the RNA preparation could be attributed to vitellogenin mRNA. Gel electrophoresis of the most purified fraction revealed residual contamination with the larger ribosomal RNA species. The molecular weight of the messenger RNA molecule, obtained by contour length measurements in the electron microscope, lies between 2.5 - 10(6) and 2.8 - 10(6)

Original language	English
Pages (from-to)	67-78
Journal	BIOCHIMICA ET BIOPHYSICA ACTA
Volume	454
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(76)90355-5
Publication status	Published - 1976

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https://doi.org/10.1016/0005-2787(76)90355-5

Cite this

@article{7b1b357ed5e24c9983dc0628d35a3bf1,

title = "ESTRADIOL-INDUCED SYNTHESIS OF VITELLOGENIN .3. ISOLATION AND CHARACTERIZATION OF VITELLOGENIN MESSENGER-RNA FROM AVIAN LIVER",

abstract = "The messenger RNA of the hormone-induced protein vitellogenin was isolated from the liver of estrogen-treated roosters. Starting from total polysomal RNA, the vitellogenin messenger was purified 67-fold by oligo (dT)-cellulose chromatography and sizing on a sucrose gradient. The messenger was translated in vitro into a 170 000 dalton polypeptide chain, having the immunochemical characteristics of vitellogenin. From electrophoretic and immunochemical analysis of the in vitro product of translation at least 63% of the messenger activity of the RNA preparation could be attributed to vitellogenin mRNA. Gel electrophoresis of the most purified fraction revealed residual contamination with the larger ribosomal RNA species. The molecular weight of the messenger RNA molecule, obtained by contour length measurements in the electron microscope, lies between 2.5 - 10(6) and 2.8 - 10(6)",

author = "G. AB and Roskam, {W. G.} and J. Dijkstra and J. Mulder and M. Willems and {van der Ende}, A. and M. Gruber",

year = "1976",

doi = "https://doi.org/10.1016/0005-2787(76)90355-5",

language = "English",

volume = "454",

pages = "67--78",

journal = "BIOCHIMICA ET BIOPHYSICA ACTA",

issn = "0006-3002",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - ESTRADIOL-INDUCED SYNTHESIS OF VITELLOGENIN .3. ISOLATION AND CHARACTERIZATION OF VITELLOGENIN MESSENGER-RNA FROM AVIAN LIVER

AU - AB, G.

AU - Roskam, W. G.

AU - Dijkstra, J.

AU - Mulder, J.

AU - Willems, M.

AU - van der Ende, A.

AU - Gruber, M.

PY - 1976

Y1 - 1976

N2 - The messenger RNA of the hormone-induced protein vitellogenin was isolated from the liver of estrogen-treated roosters. Starting from total polysomal RNA, the vitellogenin messenger was purified 67-fold by oligo (dT)-cellulose chromatography and sizing on a sucrose gradient. The messenger was translated in vitro into a 170 000 dalton polypeptide chain, having the immunochemical characteristics of vitellogenin. From electrophoretic and immunochemical analysis of the in vitro product of translation at least 63% of the messenger activity of the RNA preparation could be attributed to vitellogenin mRNA. Gel electrophoresis of the most purified fraction revealed residual contamination with the larger ribosomal RNA species. The molecular weight of the messenger RNA molecule, obtained by contour length measurements in the electron microscope, lies between 2.5 - 10(6) and 2.8 - 10(6)

AB - The messenger RNA of the hormone-induced protein vitellogenin was isolated from the liver of estrogen-treated roosters. Starting from total polysomal RNA, the vitellogenin messenger was purified 67-fold by oligo (dT)-cellulose chromatography and sizing on a sucrose gradient. The messenger was translated in vitro into a 170 000 dalton polypeptide chain, having the immunochemical characteristics of vitellogenin. From electrophoretic and immunochemical analysis of the in vitro product of translation at least 63% of the messenger activity of the RNA preparation could be attributed to vitellogenin mRNA. Gel electrophoresis of the most purified fraction revealed residual contamination with the larger ribosomal RNA species. The molecular weight of the messenger RNA molecule, obtained by contour length measurements in the electron microscope, lies between 2.5 - 10(6) and 2.8 - 10(6)

U2 - https://doi.org/10.1016/0005-2787(76)90355-5

DO - https://doi.org/10.1016/0005-2787(76)90355-5

M3 - Article

C2 - 990321

SN - 0006-3002

VL - 454

SP - 67

EP - 78

JO - BIOCHIMICA ET BIOPHYSICA ACTA

JF - BIOCHIMICA ET BIOPHYSICA ACTA

IS - 1

ER -