TY - JOUR
T1 - Evaluation of anti-human antibodies for immunohistochemistry on archival nonhuman primate tissues
AU - Wykrzykowska, Joanna J.
AU - Pauley, Douglas R.
AU - Lackner, Andrew A.
AU - Simon, Meredith A.
PY - 1996
Y1 - 1996
N2 - A panel of commercially available antibodies which recognize specific antigens on human tissues was developed for use in immunohistochemistry on tissues from eight species of nonhuman primates. Antibodies were selected for potential usefulness in diagnostic pathology, and for effectiveness in formalin-fixed, paraffin-embedded tissues. Tissues from four species of macaques and four New World monkeys were evaluated. Using these antibodies we were able to identify 17/21 antigens examined in all eight species, and 21/21 antigens in the four species of macaques. Detailed immunohistochemistry protocols are presented, along with a systematic approach to developing a protocol for a new antibody.
AB - A panel of commercially available antibodies which recognize specific antigens on human tissues was developed for use in immunohistochemistry on tissues from eight species of nonhuman primates. Antibodies were selected for potential usefulness in diagnostic pathology, and for effectiveness in formalin-fixed, paraffin-embedded tissues. Tissues from four species of macaques and four New World monkeys were evaluated. Using these antibodies we were able to identify 17/21 antigens examined in all eight species, and 21/21 antigens in the four species of macaques. Detailed immunohistochemistry protocols are presented, along with a systematic approach to developing a protocol for a new antibody.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0030112853&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/8864977
U2 - https://doi.org/10.1111/j.1600-0684.1996.tb00197.x
DO - https://doi.org/10.1111/j.1600-0684.1996.tb00197.x
M3 - Article
C2 - 8864977
SN - 0047-2565
VL - 25
SP - 71
EP - 77
JO - Journal of medical primatology
JF - Journal of medical primatology
IS - 2
ER -