Abstract
The expression of interleukin-1beta was examined in dorsal root ganglion (DRG) neurons from adult rats using non-radioactive in situ hybridization and immunocytochemistry. At all spinal levels, approximately 70% of the DRG neurons appeared to express IL-1beta mRNA; about 80% of these DRG neurons actually appeared to produce the IL-1beta protein at markedly varying levels. The expression of IL-1beta was found in large as well as in intermediate diameter sensory neurons but only sporadically in the population of small sensory neurons. The population of IL-1beta immunopositive sensory neurons included most of the large calretinin-positive Ia afferents, but only a few of the small substance P/CGRP positive sensory neurons. In situ hybridization staining for the detection of type 1 IL-1 receptor showed expression of this receptor by most of the sensory neurons as well as by supportive glial-like cells, presumably satellite cells. The functional significance of IL-1beta in the DRG neurons needs to be elucidated, but we speculate that IL-1beta produced by DRG neurons may be an auto/paracrine signalling molecule in sensory transmission
Original language | English |
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Pages (from-to) | 203-211 |
Number of pages | 9 |
Journal | Journal of Neuroimmunology |
Volume | 118 |
Issue number | 2 |
DOIs | |
Publication status | Published - 30 Aug 2001 |
Keywords
- Animals
- Calbindin 2
- Calcitonin Gene-Related Peptide
- Cell Size
- Cytoplasm
- Female
- Ganglia, Spinal
- Immunohistochemistry
- In Situ Hybridization
- Interleukin-1
- Journal Article
- Male
- Microscopy, Immunoelectron
- Neuroglia
- Neurons, Afferent
- Polyribosomes
- RNA, Messenger
- Rats
- Receptors, Interleukin-1
- Receptors, Interleukin-1 Type I
- S100 Calcium Binding Protein G
- Substance P