Factors involved in early in vitro endotoxin hyporesponsiveness in human endotoxemia

A. Nilola Kimmings; Dasja Pajkrt; Krista Zaaijer; Thybout M. Moojen; John K. Meenan; Jan W. ten Cate; Sander J.H. van Deventer

doi:https://doi.org/10.1177/096805199600300401

Factors involved in early in vitro endotoxin hyporesponsiveness in human endotoxemia

A. Nilola Kimmings, Dasja Pajkrt, Krista Zaaijer, Thybout M. Moojen, John K. Meenan, Jan W. ten Cate, Sander J.H. van Deventer

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Sepsis and endotoxemia induce a state of early tolerance to the biological effects of endotoxin. Relatively little is known about the mechanisms or mediators involved in this endotoxin hyporesponsiveness. In this study endotoxin-induced TNF production of whole blood obtained at different time points from endotoxin challenged volunteers, and endotoxin- and PHA-induced TNF production by healthy PBMNCs in the presence of serum from endotoxin challenged volunteers were compared. Endotoxin-induced TNF production by whole blood was found to be significantly reduced at 2 h after in vivo endotoxin administration, returning to baseline levels at t = 6 h. In addition, a reduction of TNF produced by healthy PBMNCs in the presence of post-endotoxin serum was observed. No correlation was found with the concentration of any of the well described anti-inflammatory mediators investigated nor with plasma levels of endotoxin-binding proteins.

Original language	English
Pages (from-to)	283-289
Number of pages	7
Journal	Innate immunity
Volume	3
Issue number	4
DOIs	https://doi.org/10.1177/096805199600300401
Publication status	Published - 1996

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title = "Factors involved in early in vitro endotoxin hyporesponsiveness in human endotoxemia",

abstract = "Sepsis and endotoxemia induce a state of early tolerance to the biological effects of endotoxin. Relatively little is known about the mechanisms or mediators involved in this endotoxin hyporesponsiveness. In this study endotoxin-induced TNF production of whole blood obtained at different time points from endotoxin challenged volunteers, and endotoxin- and PHA-induced TNF production by healthy PBMNCs in the presence of serum from endotoxin challenged volunteers were compared. Endotoxin-induced TNF production by whole blood was found to be significantly reduced at 2 h after in vivo endotoxin administration, returning to baseline levels at t = 6 h. In addition, a reduction of TNF produced by healthy PBMNCs in the presence of post-endotoxin serum was observed. No correlation was found with the concentration of any of the well described anti-inflammatory mediators investigated nor with plasma levels of endotoxin-binding proteins.",

author = "Kimmings, {A. Nilola} and Dasja Pajkrt and Krista Zaaijer and Moojen, {Thybout M.} and Meenan, {John K.} and {ten Cate}, {Jan W.} and {van Deventer}, {Sander J.H.}",

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doi = "https://doi.org/10.1177/096805199600300401",

language = "English",

volume = "3",

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T1 - Factors involved in early in vitro endotoxin hyporesponsiveness in human endotoxemia

AU - Kimmings, A. Nilola

AU - Pajkrt, Dasja

AU - Zaaijer, Krista

AU - Moojen, Thybout M.

AU - Meenan, John K.

AU - ten Cate, Jan W.

AU - van Deventer, Sander J.H.

PY - 1996

Y1 - 1996

N2 - Sepsis and endotoxemia induce a state of early tolerance to the biological effects of endotoxin. Relatively little is known about the mechanisms or mediators involved in this endotoxin hyporesponsiveness. In this study endotoxin-induced TNF production of whole blood obtained at different time points from endotoxin challenged volunteers, and endotoxin- and PHA-induced TNF production by healthy PBMNCs in the presence of serum from endotoxin challenged volunteers were compared. Endotoxin-induced TNF production by whole blood was found to be significantly reduced at 2 h after in vivo endotoxin administration, returning to baseline levels at t = 6 h. In addition, a reduction of TNF produced by healthy PBMNCs in the presence of post-endotoxin serum was observed. No correlation was found with the concentration of any of the well described anti-inflammatory mediators investigated nor with plasma levels of endotoxin-binding proteins.

AB - Sepsis and endotoxemia induce a state of early tolerance to the biological effects of endotoxin. Relatively little is known about the mechanisms or mediators involved in this endotoxin hyporesponsiveness. In this study endotoxin-induced TNF production of whole blood obtained at different time points from endotoxin challenged volunteers, and endotoxin- and PHA-induced TNF production by healthy PBMNCs in the presence of serum from endotoxin challenged volunteers were compared. Endotoxin-induced TNF production by whole blood was found to be significantly reduced at 2 h after in vivo endotoxin administration, returning to baseline levels at t = 6 h. In addition, a reduction of TNF produced by healthy PBMNCs in the presence of post-endotoxin serum was observed. No correlation was found with the concentration of any of the well described anti-inflammatory mediators investigated nor with plasma levels of endotoxin-binding proteins.

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U2 - https://doi.org/10.1177/096805199600300401

DO - https://doi.org/10.1177/096805199600300401

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SN - 1753-4259

VL - 3

SP - 283

EP - 289

JO - Innate immunity

JF - Innate immunity

IS - 4

ER -

Factors involved in early in vitro endotoxin hyporesponsiveness in human endotoxemia

Abstract

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