TY - JOUR
T1 - FLUORESCENCE LOCALIZATION IN TUMOR AND NORMAL BRAIN AFTER INTRATUMORAL INJECTION OF HEMATOPORPHYRIN DERIVATIVE INTO RAT-BRAIN TUMOR
AU - Hebeda, K. M.
AU - Wolbers, J. G.
AU - Sterenborg, H. J.
AU - Kamphorst, W.
AU - van Gemert, M. J.
AU - van Alphen, H. A.
PY - 1995
Y1 - 1995
N2 - In the intracerebral 9L rat gliosarcoma, the spatial distribution of the photosensitizer haematoporphyrin derivative (HPD) was studied after intratumoral injection. The fluorescence volume was measured in histological sections from 10 min up to 5 days after injection. Complete sensitization of the tumours could not be achieved by slow stereotactical injection of 4 mm(3) HpD (mean HpD fluorescence volume, 13+/-11 mm(3)). Larger parts of the tumour could be loaded with HpD (39+/-23 mm(3), p=0.0001) by increasing the injection velocity and the volume to 50 mm(3). Again, complete sensitization of the tumours was not achieved during a time scale of 5 days after intratumoral injection. Although the fluorescence volume did not change significantly with time, it was influenced by the injection site within the tumour. Injection of HpD within 1 mm from the tumour border resulted in significantly smaller fluorescence volumes in the tumour than injection into the tumour centre. Large injection volumes caused an increased leakage of HpD to normal brain, leading to the loss of selectivity of photosensitizer content and the occurrence of dark toxicity of normal brain while the tumours still appeared vital
AB - In the intracerebral 9L rat gliosarcoma, the spatial distribution of the photosensitizer haematoporphyrin derivative (HPD) was studied after intratumoral injection. The fluorescence volume was measured in histological sections from 10 min up to 5 days after injection. Complete sensitization of the tumours could not be achieved by slow stereotactical injection of 4 mm(3) HpD (mean HpD fluorescence volume, 13+/-11 mm(3)). Larger parts of the tumour could be loaded with HpD (39+/-23 mm(3), p=0.0001) by increasing the injection velocity and the volume to 50 mm(3). Again, complete sensitization of the tumours was not achieved during a time scale of 5 days after intratumoral injection. Although the fluorescence volume did not change significantly with time, it was influenced by the injection site within the tumour. Injection of HpD within 1 mm from the tumour border resulted in significantly smaller fluorescence volumes in the tumour than injection into the tumour centre. Large injection volumes caused an increased leakage of HpD to normal brain, leading to the loss of selectivity of photosensitizer content and the occurrence of dark toxicity of normal brain while the tumours still appeared vital
U2 - https://doi.org/10.1016/1011-1344(94)07056-T
DO - https://doi.org/10.1016/1011-1344(94)07056-T
M3 - Article
C2 - 7699526
SN - 1011-1344
VL - 27
SP - 85
EP - 92
JO - Journal of photochemistry and photobiology. B, Biology
JF - Journal of photochemistry and photobiology. B, Biology
IS - 1
ER -