Fluorimetric determination of carbamoyl phosphate

R. J. Wanders; C. van Roermund; C. Lof; A. J. Meijer

doi:https://doi.org/10.1016/0003-2697(83)90055-6

Fluorimetric determination of carbamoyl phosphate

R. J. Wanders, C. van Roermund, C. Lof, A. J. Meijer

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Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A simple fluorimetric assay for the determination of carbamoyl phosphate in tissue extracts is described. In the assay, production of ATP from carbamoyl phosphate and ADP by carbamate kinase is coupled to the formation of NADPH, using glucose, hexokinase, NADP+, and glucose-6-phosphate dehydrogenase. Production of NADPH in this system proved to be equal to the amount of carbamoyl phosphate present

Original language	English
Pages (from-to)	80-87
Journal	Analytical biochemistry
Volume	129
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(83)90055-6
Publication status	Published - 1983

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https://doi.org/10.1016/0003-2697(83)90055-6

Cite this

@article{c4bdcddd6f2b4dd18f1f72fc1279db49,

title = "Fluorimetric determination of carbamoyl phosphate",

abstract = "A simple fluorimetric assay for the determination of carbamoyl phosphate in tissue extracts is described. In the assay, production of ATP from carbamoyl phosphate and ADP by carbamate kinase is coupled to the formation of NADPH, using glucose, hexokinase, NADP+, and glucose-6-phosphate dehydrogenase. Production of NADPH in this system proved to be equal to the amount of carbamoyl phosphate present",

author = "Wanders, {R. J.} and {van Roermund}, C. and C. Lof and Meijer, {A. J.}",

year = "1983",

doi = "https://doi.org/10.1016/0003-2697(83)90055-6",

language = "English",

volume = "129",

pages = "80--87",

journal = "Analytical biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Fluorimetric determination of carbamoyl phosphate

AU - Wanders, R. J.

AU - van Roermund, C.

AU - Lof, C.

AU - Meijer, A. J.

PY - 1983

Y1 - 1983

N2 - A simple fluorimetric assay for the determination of carbamoyl phosphate in tissue extracts is described. In the assay, production of ATP from carbamoyl phosphate and ADP by carbamate kinase is coupled to the formation of NADPH, using glucose, hexokinase, NADP+, and glucose-6-phosphate dehydrogenase. Production of NADPH in this system proved to be equal to the amount of carbamoyl phosphate present

AB - A simple fluorimetric assay for the determination of carbamoyl phosphate in tissue extracts is described. In the assay, production of ATP from carbamoyl phosphate and ADP by carbamate kinase is coupled to the formation of NADPH, using glucose, hexokinase, NADP+, and glucose-6-phosphate dehydrogenase. Production of NADPH in this system proved to be equal to the amount of carbamoyl phosphate present

U2 - https://doi.org/10.1016/0003-2697(83)90055-6

DO - https://doi.org/10.1016/0003-2697(83)90055-6

M3 - Article

C2 - 6859536

SN - 0003-2697

VL - 129

SP - 80

EP - 87

JO - Analytical biochemistry

JF - Analytical biochemistry

IS - 1

ER -