TY - JOUR
T1 - Food allergen sensitization on a chip
T2 - the gut–immune–skin axis
AU - Janssen, Robine
AU - de Kleer, Janna W. M.
AU - Heming, Bo
AU - Bastiaan-Net, Shanna
AU - Garssen, Johan
AU - Willemsen, Linette E. M.
AU - Masereeuw, Rosalinde
N1 - Publisher Copyright: © 2023 The Author(s)
PY - 2024/1
Y1 - 2024/1
N2 - The global population is growing, rapidly increasing the demand for sustainable, novel, and safe food proteins with minimal risks of food allergy. In vitro testing of allergy-sensitizing capacity is predominantly based on 2D assays. However, these lack the 3D environment and crosstalk between the gut, skin, and immune cells essential for allergy prediction. Organ-on-a-chip (OoC) technologies are promising to study type 2 immune activation required for sensitization, initiated in the small intestine or skin, in interlinked systems. Increasing the mechanistic understanding and, moreover, finding new strategies to study interorgan communication is of importance to recapitulate food allergen sensitization in vitro. Here, we outline recently developed OoC platforms and discuss the features needed for reliable prediction of sensitizing allergenicity of proteins.
AB - The global population is growing, rapidly increasing the demand for sustainable, novel, and safe food proteins with minimal risks of food allergy. In vitro testing of allergy-sensitizing capacity is predominantly based on 2D assays. However, these lack the 3D environment and crosstalk between the gut, skin, and immune cells essential for allergy prediction. Organ-on-a-chip (OoC) technologies are promising to study type 2 immune activation required for sensitization, initiated in the small intestine or skin, in interlinked systems. Increasing the mechanistic understanding and, moreover, finding new strategies to study interorgan communication is of importance to recapitulate food allergen sensitization in vitro. Here, we outline recently developed OoC platforms and discuss the features needed for reliable prediction of sensitizing allergenicity of proteins.
KW - food allergen sensitization
KW - gut–immune–skin axis
KW - microfluidics
KW - organ-on-a-chip technologies
UR - http://www.scopus.com/inward/record.url?scp=85168385494&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.tibtech.2023.07.005
DO - https://doi.org/10.1016/j.tibtech.2023.07.005
M3 - Review article
C2 - 37580191
SN - 0167-7799
VL - 42
SP - 119
EP - 134
JO - Trends in biotechnology
JF - Trends in biotechnology
IS - 1
ER -