From fixed to FRAP: measuring protein mobility and activity in living cells

E. A. Reits; J. J. Neefjes

doi:https://doi.org/10.1038/35078615

From fixed to FRAP: measuring protein mobility and activity in living cells

E. A. Reits, J. J. Neefjes

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Research output: Contribution to journal › Review article › Academic › peer-review

528 Citations (Scopus)

Abstract

Experiments with fluorescence recovery after photobleaching (FRAP) started 30 years ago to visualize the lateral mobility and dynamics of fluorescent proteins in living cells. Its popularity increased when non-invasive fluorescent tagging became possible with the green fluorescent protein (GFP). Many researchers use GFP to study the localization of fusion proteins in fixed or living cells, but the same fluorescent proteins can also be used to study protein mobility in living cells. Here we review the potential of FRAP to study protein dynamics and activity within a single living cell. These measurements can be made with most standard confocal laser-scanning microscopes equipped with photobleaching protocols

Original language	English
Pages (from-to)	E145-E147
Journal	Nature cell biology
Volume	3
Issue number	6
DOIs	https://doi.org/10.1038/35078615
Publication status	Published - 2001

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https://doi.org/10.1038/35078615

Cite this

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title = "From fixed to FRAP: measuring protein mobility and activity in living cells",

abstract = "Experiments with fluorescence recovery after photobleaching (FRAP) started 30 years ago to visualize the lateral mobility and dynamics of fluorescent proteins in living cells. Its popularity increased when non-invasive fluorescent tagging became possible with the green fluorescent protein (GFP). Many researchers use GFP to study the localization of fusion proteins in fixed or living cells, but the same fluorescent proteins can also be used to study protein mobility in living cells. Here we review the potential of FRAP to study protein dynamics and activity within a single living cell. These measurements can be made with most standard confocal laser-scanning microscopes equipped with photobleaching protocols",

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AB - Experiments with fluorescence recovery after photobleaching (FRAP) started 30 years ago to visualize the lateral mobility and dynamics of fluorescent proteins in living cells. Its popularity increased when non-invasive fluorescent tagging became possible with the green fluorescent protein (GFP). Many researchers use GFP to study the localization of fusion proteins in fixed or living cells, but the same fluorescent proteins can also be used to study protein mobility in living cells. Here we review the potential of FRAP to study protein dynamics and activity within a single living cell. These measurements can be made with most standard confocal laser-scanning microscopes equipped with photobleaching protocols

U2 - https://doi.org/10.1038/35078615

DO - https://doi.org/10.1038/35078615

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