TY - JOUR
T1 - Gene and cluster-specific expression of the Iroquois family members during mouse development
AU - Houweling, A. C.
AU - Dildrop, R.
AU - Peters, T.
AU - Mummenhoff, J.
AU - Moorman, A. F.
AU - Rüther, U.
AU - Christoffels, V. M.
N1 - Funding Information: We thank Piet A.J. de Boer and Danielle E.W. Clout for expert technical assistance, Frits de Jong for stimulating discussions, Astrid G.M. Keijser, Lars Grotewold and Jan M. Ruijter for their contributions. A.C.H., A.F.M.M. and V.M.C. are supported by the Netherlands Heart Foundation project M96.002. U.R. is supported by the Deutsche Forschungsgemeinschaft (Ru 376/8).
PY - 2001
Y1 - 2001
N2 - Mammalian homologues of the Drosophila Iroquois homeobox gene complex, involved in patterning and regionalization of differentiation, have recently been identified (Mech. Dev., 69 (1997) 169; Dev. Biol., 217 (2000) 266; Dev. Dyn., 218 (2000) 160; Mech. Dev., 91 (2000) 317; Dev. Biol., 224 (2000) 263; Genome Res., 10 (2000) 1453; Mech. Dev., 103 (2001) 193). The six members of the murine family were found to be organized in two cognate clusters of three genes each, Irx1, -2, -4 and Irx3, -5, -6, respectively (Peters et al., 2000). As a basis for further study of their regulation and function we performed a comparative analysis of the genomic organization and of the expression patterns of all six Irx genes. The genes are expressed in highly specific and regionalized patterns of ectoderm, mesoderm and endoderm derived tissues. In most tissues the pattern of expression of the clustered genes, especially of Irx1 and -2 and of Irx3 and -5, respectively, closely resembled each other while those of Irx4 and -6 were very divergent. Interestingly, the expression of cognate genes was found to be mutually exclusive in adjacent and interacting tissues of limb, heart and the laryncho-pharyncheal region. The results indicate that the Irx genes are coordinately regulated at the level of the cluster
AB - Mammalian homologues of the Drosophila Iroquois homeobox gene complex, involved in patterning and regionalization of differentiation, have recently been identified (Mech. Dev., 69 (1997) 169; Dev. Biol., 217 (2000) 266; Dev. Dyn., 218 (2000) 160; Mech. Dev., 91 (2000) 317; Dev. Biol., 224 (2000) 263; Genome Res., 10 (2000) 1453; Mech. Dev., 103 (2001) 193). The six members of the murine family were found to be organized in two cognate clusters of three genes each, Irx1, -2, -4 and Irx3, -5, -6, respectively (Peters et al., 2000). As a basis for further study of their regulation and function we performed a comparative analysis of the genomic organization and of the expression patterns of all six Irx genes. The genes are expressed in highly specific and regionalized patterns of ectoderm, mesoderm and endoderm derived tissues. In most tissues the pattern of expression of the clustered genes, especially of Irx1 and -2 and of Irx3 and -5, respectively, closely resembled each other while those of Irx4 and -6 were very divergent. Interestingly, the expression of cognate genes was found to be mutually exclusive in adjacent and interacting tissues of limb, heart and the laryncho-pharyncheal region. The results indicate that the Irx genes are coordinately regulated at the level of the cluster
KW - Cluster
KW - Cognate
KW - Coordinated regulation
KW - Evolutionary conservation
KW - Heart
KW - Iroquois homeobox genes
KW - Irx
KW - Limb
KW - Lung
KW - Mammary gland
KW - Nervous system
KW - Organogenesis
KW - Tooth
KW - Vibrissae
UR - http://www.scopus.com/inward/record.url?scp=0034891325&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/S0925-4773(01)00451-8
DO - https://doi.org/10.1016/S0925-4773(01)00451-8
M3 - Article
C2 - 11520674
SN - 0925-4773
VL - 107
SP - 169
EP - 174
JO - Mechanisms of development
JF - Mechanisms of development
IS - 1-2
ER -