Abstract
A method is described for the fast and homogeneous increase of the temperature of a biological specimen with a volume of 1 mm3 or less. Heating is obtained by joule dissipation from a 30 MHz alternating current. The step quality is determined by measuring the temporal and spatial behaviour of the temperature after a power pulse in and around the biological preparation. With the described equipment a temperature step of 3 degrees C can be obtained in 8 ms. The temperature step inhomogeneity is less than 15%. The compatibility of the method with electrophysiological and mechanical measurement and instrumentation is demonstrated by the detection of transients in transmembrane potential and force of a papillary muscle from the rabbit heart
| Original language | English |
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| Pages (from-to) | 233-242 |
| Journal | Journal of Biochemical and Biophysical Methods |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1987 |