TY - JOUR
T1 - Generation of a human induced pluripotent stem cell line (MUSIi001-A) from caesarean section scar fibroblasts using Sendai viral vectors
AU - Wattanapanitch, Methichit
AU - Ritthaphai, Alisa
AU - Park, Chanmi
AU - Boonkaew, Bootsakorn
AU - Netsrithong, Ratchapong
AU - Pattanapanyasat, Kovit
AU - Limsiri, Pattarawan
AU - Vatanashevanopakorn, Chinnavuth
N1 - Funding Information: This research project was supported by the Thailand Research Fund (TRF) – Distinguished Research Professor Grant, contract no. DPG5980001 and IRG 5980006 and the Siriraj Research Fund, Faculty of Medicine Siriraj Hospital, Mahidol University , Grant number (IO) R016033017 and R015933012 . MW, PL and CV are supported by Chalermphrakiat Grant, Faculty of Medicine Siriraj Hospital, Mahidol University . AR is supported by Siriraj Graduate Scholarship, Faculty of Medicine Siriraj Hospital, Mahidol University . Publisher Copyright: © 2018 The Authors
PY - 2018/3
Y1 - 2018/3
N2 - We generated a human induced pluripotent stem cell (iPSC) line from caesarean section scar fibroblasts of a 33-year-old healthy woman using transgene-free Sendai viral vectors under feeder-free condition. The established iPSC line, designated as MUSIi001-A, exhibited a normal karyotype, expressed pluripotent markers, differentiated into cells of three embryonic germ layers. Further analyses showed that the Sendai viral genome was absent at passage 25. The MUSIi001-A line can serve as a control for studying developmental biology and phenotypic comparison with disease-specific iPSCs.
AB - We generated a human induced pluripotent stem cell (iPSC) line from caesarean section scar fibroblasts of a 33-year-old healthy woman using transgene-free Sendai viral vectors under feeder-free condition. The established iPSC line, designated as MUSIi001-A, exhibited a normal karyotype, expressed pluripotent markers, differentiated into cells of three embryonic germ layers. Further analyses showed that the Sendai viral genome was absent at passage 25. The MUSIi001-A line can serve as a control for studying developmental biology and phenotypic comparison with disease-specific iPSCs.
UR - http://www.scopus.com/inward/record.url?scp=85041465774&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.scr.2018.01.013
DO - https://doi.org/10.1016/j.scr.2018.01.013
M3 - Article
C2 - 29367084
SN - 1873-5061
VL - 27
SP - 105
EP - 108
JO - Stem Cell Research
JF - Stem Cell Research
ER -