Generation of Fibrodysplasia ossificans progressiva and control integration free iPSC lines from periodontal ligament fibroblasts

G. Sanchez-Duffhues; H. Mikkers; D. de Jong; K. Szuhai; T. J. de Vries; C. Freund; N. Bravenboer; R. J.J. van Es; J. C. Netelenbos; M. J. Goumans; E. M.W. Eekhoff; P. ten Dijke

doi:https://doi.org/10.1016/j.scr.2019.101639

Generation of Fibrodysplasia ossificans progressiva and control integration free iPSC lines from periodontal ligament fibroblasts

G. Sanchez-Duffhues, H. Mikkers, D. de Jong, K. Szuhai, T. J. de Vries, C. Freund, N. Bravenboer, R. J.J. van Es, J. C. Netelenbos, M. J. Goumans, E. M.W. Eekhoff, P. ten Dijke

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

Fibrodysplasia ossificans progressiva (FOP) is a very rare devastating heterotopic ossification disorder, classically caused by a heterozygous single point mutation (c.617G>A) in the ACVR1gene, encoding the Bone morphogenetic protein (BMP) type I receptor, also termed activin receptor-like kinase (ALK)2. FOP patients develop heterotopic ossification episodically in response to inflammatory insults, thereby compromising tissue sampling and the development of in vitro surrogate models for FOP. Here we describe the generation and characterization of a control and a classical FOP induced pluripotent stem cell (iPSC) line derived from periodontal ligament fibroblast cells using Sendai virus vectors.

Original language	English
Article number	101639
Number of pages	4
Journal	Stem Cell Research
Volume	41
DOIs	https://doi.org/10.1016/j.scr.2019.101639
Publication status	Published - Dec 2019

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Sanchez-Duffhues, G., Mikkers, H., de Jong, D., Szuhai, K., de Vries, T. J., Freund, C., Bravenboer, N., van Es, R. J. J., Netelenbos, J. C., Goumans, M. J., Eekhoff, E. M. W., & ten Dijke, P. (2019). Generation of Fibrodysplasia ossificans progressiva and control integration free iPSC lines from periodontal ligament fibroblasts. Stem Cell Research, 41, Article 101639. https://doi.org/10.1016/j.scr.2019.101639

@article{c7885008b55e4a9bb04dfe1bfa051771,

title = "Generation of Fibrodysplasia ossificans progressiva and control integration free iPSC lines from periodontal ligament fibroblasts",

abstract = "Fibrodysplasia ossificans progressiva (FOP) is a very rare devastating heterotopic ossification disorder, classically caused by a heterozygous single point mutation (c.617G>A) in the ACVR1gene, encoding the Bone morphogenetic protein (BMP) type I receptor, also termed activin receptor-like kinase (ALK)2. FOP patients develop heterotopic ossification episodically in response to inflammatory insults, thereby compromising tissue sampling and the development of in vitro surrogate models for FOP. Here we describe the generation and characterization of a control and a classical FOP induced pluripotent stem cell (iPSC) line derived from periodontal ligament fibroblast cells using Sendai virus vectors.",

author = "G. Sanchez-Duffhues and H. Mikkers and {de Jong}, D. and K. Szuhai and {de Vries}, {T. J.} and C. Freund and N. Bravenboer and {van Es}, {R. J.J.} and Netelenbos, {J. C.} and Goumans, {M. J.} and Eekhoff, {E. M.W.} and {ten Dijke}, P.",

note = "Publisher Copyright: {\textcopyright} 2019 The Authors",

year = "2019",

month = dec,

doi = "https://doi.org/10.1016/j.scr.2019.101639",

language = "English",

volume = "41",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

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Sanchez-Duffhues, G, Mikkers, H, de Jong, D, Szuhai, K, de Vries, TJ, Freund, C, Bravenboer, N, van Es, RJJ, Netelenbos, JC, Goumans, MJ, Eekhoff, EMW & ten Dijke, P 2019, 'Generation of Fibrodysplasia ossificans progressiva and control integration free iPSC lines from periodontal ligament fibroblasts', Stem Cell Research, vol. 41, 101639. https://doi.org/10.1016/j.scr.2019.101639

TY - JOUR

T1 - Generation of Fibrodysplasia ossificans progressiva and control integration free iPSC lines from periodontal ligament fibroblasts

AU - Sanchez-Duffhues, G.

AU - Mikkers, H.

AU - de Jong, D.

AU - Szuhai, K.

AU - de Vries, T. J.

AU - Freund, C.

AU - Bravenboer, N.

AU - van Es, R. J.J.

AU - Netelenbos, J. C.

AU - Goumans, M. J.

AU - Eekhoff, E. M.W.

AU - ten Dijke, P.

PY - 2019/12

Y1 - 2019/12

N2 - Fibrodysplasia ossificans progressiva (FOP) is a very rare devastating heterotopic ossification disorder, classically caused by a heterozygous single point mutation (c.617G>A) in the ACVR1gene, encoding the Bone morphogenetic protein (BMP) type I receptor, also termed activin receptor-like kinase (ALK)2. FOP patients develop heterotopic ossification episodically in response to inflammatory insults, thereby compromising tissue sampling and the development of in vitro surrogate models for FOP. Here we describe the generation and characterization of a control and a classical FOP induced pluripotent stem cell (iPSC) line derived from periodontal ligament fibroblast cells using Sendai virus vectors.

AB - Fibrodysplasia ossificans progressiva (FOP) is a very rare devastating heterotopic ossification disorder, classically caused by a heterozygous single point mutation (c.617G>A) in the ACVR1gene, encoding the Bone morphogenetic protein (BMP) type I receptor, also termed activin receptor-like kinase (ALK)2. FOP patients develop heterotopic ossification episodically in response to inflammatory insults, thereby compromising tissue sampling and the development of in vitro surrogate models for FOP. Here we describe the generation and characterization of a control and a classical FOP induced pluripotent stem cell (iPSC) line derived from periodontal ligament fibroblast cells using Sendai virus vectors.

UR - http://www.scopus.com/inward/record.url?scp=85074704758&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.scr.2019.101639

DO - https://doi.org/10.1016/j.scr.2019.101639

M3 - Article

C2 - 31733439

SN - 1873-5061

VL - 41

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101639

ER -

Generation of Fibrodysplasia ossificans progressiva and control integration free iPSC lines from periodontal ligament fibroblasts

Abstract

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