TY - JOUR
T1 - Generation of two induced pluripotent stem cell lines (MUSIi011-A and MUSIi011-B) from peripheral blood T lymphocytes of a healthy individual
AU - Netsrithong, Ratchapong
AU - Promnakhon, Nutchanawan
AU - Boonkaew, Bootsakorn
AU - Vatanashevanopakorn, Chinnavuth
AU - Pattanapanyasat, Kovit
AU - Wattanapanitch, Methichit
N1 - Funding Information: This study was supported by Thailand Research Fund (grant no. RSA6280090 , DPG5980001 and IRG5980006 ) and the Siriraj Research Fund, Faculty of Medicine Siriraj Hospital, Mahidol University, (grant no. (IO) R016234002 ). RN is supported by the Development and Promotion of Science and Technology Talents Project. NP is supported by the Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program and Faculty of Medicine Siriraj Hospital, Mahidol University (grant no. PHD/0203/2556 ). CV and MW are supported by Chalermphrakiat Grant, Faculty of Medicine Siriraj Hospital, Mahidol University . Funding Information: This study was supported by Thailand Research Fund (grant no. RSA6280090, DPG5980001 and IRG5980006) and the Siriraj Research Fund, Faculty of Medicine Siriraj Hospital, Mahidol University, (grant no. (IO) R016234002). RN is supported by the Development and Promotion of Science and Technology Talents Project. NP is supported by the Thailand Research Fund through the Royal Golden Jubilee Ph.D. Program and Faculty of Medicine Siriraj Hospital, Mahidol University (grant no. PHD/0203/2556). CV and MW are supported by Chalermphrakiat Grant, Faculty of Medicine Siriraj Hospital, Mahidol University. Publisher Copyright: © 2019
PY - 2019/8
Y1 - 2019/8
N2 - Activated T lymphocytes of a healthy individual were reprogrammed to induced pluripotent stem cells (iPSCs) using Sendai viral vectors. Two iPSC lines, MUSIi011-A and MUSIi011-B, were established and characterized for the expression of pluripotent markers. Both iPSC lines were able to differentiate into cells of three embryonic germ layers via embryoid body formation, exhibited normal karyotypes and were free of viral genome and transgenes at passage 15. These T lymphocyte-derived iPSCs (T-iPSCs) represent a useful starting cell source for developing next-generation immune cells such as chimeric antigen receptor (CAR)-engineered iPSC-derived T lymphocytes for the application in adoptive immunotherapy.
AB - Activated T lymphocytes of a healthy individual were reprogrammed to induced pluripotent stem cells (iPSCs) using Sendai viral vectors. Two iPSC lines, MUSIi011-A and MUSIi011-B, were established and characterized for the expression of pluripotent markers. Both iPSC lines were able to differentiate into cells of three embryonic germ layers via embryoid body formation, exhibited normal karyotypes and were free of viral genome and transgenes at passage 15. These T lymphocyte-derived iPSCs (T-iPSCs) represent a useful starting cell source for developing next-generation immune cells such as chimeric antigen receptor (CAR)-engineered iPSC-derived T lymphocytes for the application in adoptive immunotherapy.
UR - http://www.scopus.com/inward/record.url?scp=85067458253&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.scr.2019.101487
DO - https://doi.org/10.1016/j.scr.2019.101487
M3 - Article
C2 - 31229899
SN - 1873-5061
VL - 39
JO - Stem Cell Research
JF - Stem Cell Research
M1 - 101487
ER -