Glucose-6-phosphate dehydrogenase activity decreases during storage of leukoreduced red blood cells

Anna L. Peters; Robin van Bruggen; Dirk de Korte; Cornelis J. F. van Noorden; Alexander P. J. Vlaar

doi:https://doi.org/10.1111/trf.13378

Glucose-6-phosphate dehydrogenase activity decreases during storage of leukoreduced red blood cells

Anna L. Peters, Robin van Bruggen, Dirk de Korte, Cornelis J. F. van Noorden, Alexander P. J. Vlaar

Research output: Contribution to journal › Article › Academic › peer-review

21 Citations (Scopus)

Abstract

During storage, the activity of the red blood cell (RBC) antioxidant system decreases. Glucose-6-phosphate dehydrogenase (G6PD) is essential for protection against oxidative stress by producing NADPH. G6PD function of RBC transfusion products is reported to remain stable during storage, but activity was measured in hemolysates and not in individual RBCs. We hypothesized that analysis of G6PD activity in individual RBC identifies storage-dependent changes in G6PD function. Seven units of stored leukoreduced RBCs, stored in saline-adenine-glucose-mannitol, were sampled every week up to 6 weeks of storage. G6PD activity was determined with the cytofluorometric method and expressed as mean fluorescent intensity (MFI) per RBC. During storage, G6PD activity decreased significantly. Mean MFI after 3 days of storage was 27.8 ± 8.8 and gradually decreased significantly to 18.0 ± 8.3 after 42 days. G6PD activity decreases during storage of leukoreduced RBCs. Our results may form a new target to improve storage conditions of RBCs and subsequently improve the quality of transfusion products

Original language	English
Pages (from-to)	427-432
Journal	Transfusion
Volume	56
Issue number	2
DOIs	https://doi.org/10.1111/trf.13378
Publication status	Published - 2016

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https://doi.org/10.1111/trf.13378

Cite this

@article{4d5b7f1bfa814ec080068285edbd3914,

title = "Glucose-6-phosphate dehydrogenase activity decreases during storage of leukoreduced red blood cells",

abstract = "During storage, the activity of the red blood cell (RBC) antioxidant system decreases. Glucose-6-phosphate dehydrogenase (G6PD) is essential for protection against oxidative stress by producing NADPH. G6PD function of RBC transfusion products is reported to remain stable during storage, but activity was measured in hemolysates and not in individual RBCs. We hypothesized that analysis of G6PD activity in individual RBC identifies storage-dependent changes in G6PD function. Seven units of stored leukoreduced RBCs, stored in saline-adenine-glucose-mannitol, were sampled every week up to 6 weeks of storage. G6PD activity was determined with the cytofluorometric method and expressed as mean fluorescent intensity (MFI) per RBC. During storage, G6PD activity decreased significantly. Mean MFI after 3 days of storage was 27.8 ± 8.8 and gradually decreased significantly to 18.0 ± 8.3 after 42 days. G6PD activity decreases during storage of leukoreduced RBCs. Our results may form a new target to improve storage conditions of RBCs and subsequently improve the quality of transfusion products",

author = "Peters, {Anna L.} and {van Bruggen}, Robin and {de Korte}, Dirk and {van Noorden}, {Cornelis J. F.} and Vlaar, {Alexander P. J.}",

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T1 - Glucose-6-phosphate dehydrogenase activity decreases during storage of leukoreduced red blood cells

AU - Peters, Anna L.

AU - van Bruggen, Robin

AU - de Korte, Dirk

AU - van Noorden, Cornelis J. F.

AU - Vlaar, Alexander P. J.

PY - 2016

Y1 - 2016

N2 - During storage, the activity of the red blood cell (RBC) antioxidant system decreases. Glucose-6-phosphate dehydrogenase (G6PD) is essential for protection against oxidative stress by producing NADPH. G6PD function of RBC transfusion products is reported to remain stable during storage, but activity was measured in hemolysates and not in individual RBCs. We hypothesized that analysis of G6PD activity in individual RBC identifies storage-dependent changes in G6PD function. Seven units of stored leukoreduced RBCs, stored in saline-adenine-glucose-mannitol, were sampled every week up to 6 weeks of storage. G6PD activity was determined with the cytofluorometric method and expressed as mean fluorescent intensity (MFI) per RBC. During storage, G6PD activity decreased significantly. Mean MFI after 3 days of storage was 27.8 ± 8.8 and gradually decreased significantly to 18.0 ± 8.3 after 42 days. G6PD activity decreases during storage of leukoreduced RBCs. Our results may form a new target to improve storage conditions of RBCs and subsequently improve the quality of transfusion products

AB - During storage, the activity of the red blood cell (RBC) antioxidant system decreases. Glucose-6-phosphate dehydrogenase (G6PD) is essential for protection against oxidative stress by producing NADPH. G6PD function of RBC transfusion products is reported to remain stable during storage, but activity was measured in hemolysates and not in individual RBCs. We hypothesized that analysis of G6PD activity in individual RBC identifies storage-dependent changes in G6PD function. Seven units of stored leukoreduced RBCs, stored in saline-adenine-glucose-mannitol, were sampled every week up to 6 weeks of storage. G6PD activity was determined with the cytofluorometric method and expressed as mean fluorescent intensity (MFI) per RBC. During storage, G6PD activity decreased significantly. Mean MFI after 3 days of storage was 27.8 ± 8.8 and gradually decreased significantly to 18.0 ± 8.3 after 42 days. G6PD activity decreases during storage of leukoreduced RBCs. Our results may form a new target to improve storage conditions of RBCs and subsequently improve the quality of transfusion products

U2 - https://doi.org/10.1111/trf.13378

DO - https://doi.org/10.1111/trf.13378

M3 - Article

C2 - 26456480

SN - 0041-1132

VL - 56

SP - 427

EP - 432

JO - Transfusion

JF - Transfusion

IS - 2

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