Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level

Iris E. Allijn; Wei Leong; Jun Tang; Anita Gianella; Aneta J. Mieszawska; Francois Fay; Ge Ma; Stewart Russell; Catherine B. Callo; Ronald E. Gordon; Emine Korkmaz; Jan Andries Post; Yiming Zhao; Hans C. Gerritsen; Axel Thran; Roland Proksa; Heiner Daerr; Gert Storm; Valentin Fuster; Edward A. Fisher; Zahi A. Fayad; Willem J. M. Mulder; David P. Cormode

doi:https://doi.org/10.1021/nn403258w

Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level

Iris E. Allijn, Wei Leong, Jun Tang, Anita Gianella, Aneta J. Mieszawska, Francois Fay, Ge Ma, Stewart Russell, Catherine B. Callo, Ronald E. Gordon, Emine Korkmaz, Jan Andries Post, Yiming Zhao, Hans C. Gerritsen, Axel Thran, Roland Proksa, Heiner Daerr, Gert Storm, Valentin Fuster, Edward A. FisherZahi A. Fayad, Willem J. M. Mulder, David P. Cormode

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Abstract

Low-density lipoprotein (LDL) plays a critical role in cholesterol transport and is closely linked to the progression of several diseases. This motivates the development of methods to study LDL behavior from the microscopic to whole-body level. We have developed an approach to efficiently load LDL with a range of diagnostically active nanocrystals or hydrophobic agents. We performed focused experiments on LDL labeled with gold nanocrystals (Au-LDL). The labeling procedure had minimal effect on LDL size, morphology, or composition. Biological function was found to be maintained from both in vitro and in vivo experiments. Tumor-bearing mice were injected intravenously with LDL, DiR-LDL, Au-LDL, or a gold-loaded nanoemulsion. LDL accumulation in the tumors was detected with whole-body imaging methods, such as computed tomography (CT), spectral CT, and fluorescence imaging. Cellular localization was studied with transmission electron microscopy and fluorescence techniques. This LDL labeling procedure should permit the study of lipoprotein biointeractions in unprecedented detail

Original language	English
Pages (from-to)	9761-9770
Journal	ACS nano
Volume	7
Issue number	11
DOIs	https://doi.org/10.1021/nn403258w
Publication status	Published - 2013

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https://doi.org/10.1021/nn403258w

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Allijn, I. E., Leong, W., Tang, J., Gianella, A., Mieszawska, A. J., Fay, F., Ma, G., Russell, S., Callo, C. B., Gordon, R. E., Korkmaz, E., Post, J. A., Zhao, Y., Gerritsen, H. C., Thran, A., Proksa, R., Daerr, H., Storm, G., Fuster, V., ... Cormode, D. P. (2013). Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level. ACS nano, 7(11), 9761-9770. https://doi.org/10.1021/nn403258w

@article{a2ab904b8a904a009546d4fde5fbe16c,

title = "Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level",

abstract = "Low-density lipoprotein (LDL) plays a critical role in cholesterol transport and is closely linked to the progression of several diseases. This motivates the development of methods to study LDL behavior from the microscopic to whole-body level. We have developed an approach to efficiently load LDL with a range of diagnostically active nanocrystals or hydrophobic agents. We performed focused experiments on LDL labeled with gold nanocrystals (Au-LDL). The labeling procedure had minimal effect on LDL size, morphology, or composition. Biological function was found to be maintained from both in vitro and in vivo experiments. Tumor-bearing mice were injected intravenously with LDL, DiR-LDL, Au-LDL, or a gold-loaded nanoemulsion. LDL accumulation in the tumors was detected with whole-body imaging methods, such as computed tomography (CT), spectral CT, and fluorescence imaging. Cellular localization was studied with transmission electron microscopy and fluorescence techniques. This LDL labeling procedure should permit the study of lipoprotein biointeractions in unprecedented detail",

author = "Allijn, {Iris E.} and Wei Leong and Jun Tang and Anita Gianella and Mieszawska, {Aneta J.} and Francois Fay and Ge Ma and Stewart Russell and Callo, {Catherine B.} and Gordon, {Ronald E.} and Emine Korkmaz and Post, {Jan Andries} and Yiming Zhao and Gerritsen, {Hans C.} and Axel Thran and Roland Proksa and Heiner Daerr and Gert Storm and Valentin Fuster and Fisher, {Edward A.} and Fayad, {Zahi A.} and Mulder, {Willem J. M.} and Cormode, {David P.}",

year = "2013",

doi = "https://doi.org/10.1021/nn403258w",

language = "English",

volume = "7",

pages = "9761--9770",

journal = "ACS nano",

issn = "1936-0851",

publisher = "American Chemical Society",

number = "11",

}

Allijn, IE, Leong, W, Tang, J, Gianella, A, Mieszawska, AJ, Fay, F, Ma, G, Russell, S, Callo, CB, Gordon, RE, Korkmaz, E, Post, JA, Zhao, Y, Gerritsen, HC, Thran, A, Proksa, R, Daerr, H, Storm, G, Fuster, V, Fisher, EA, Fayad, ZA, Mulder, WJM & Cormode, DP 2013, 'Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level', ACS nano, vol. 7, no. 11, pp. 9761-9770. https://doi.org/10.1021/nn403258w

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AU - Allijn, Iris E.

AU - Leong, Wei

AU - Tang, Jun

AU - Gianella, Anita

AU - Mieszawska, Aneta J.

AU - Fay, Francois

AU - Ma, Ge

AU - Russell, Stewart

AU - Callo, Catherine B.

AU - Gordon, Ronald E.

AU - Korkmaz, Emine

AU - Post, Jan Andries

AU - Zhao, Yiming

AU - Gerritsen, Hans C.

AU - Thran, Axel

AU - Proksa, Roland

AU - Daerr, Heiner

AU - Storm, Gert

AU - Fuster, Valentin

AU - Fisher, Edward A.

AU - Fayad, Zahi A.

AU - Mulder, Willem J. M.

AU - Cormode, David P.

PY - 2013

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N2 - Low-density lipoprotein (LDL) plays a critical role in cholesterol transport and is closely linked to the progression of several diseases. This motivates the development of methods to study LDL behavior from the microscopic to whole-body level. We have developed an approach to efficiently load LDL with a range of diagnostically active nanocrystals or hydrophobic agents. We performed focused experiments on LDL labeled with gold nanocrystals (Au-LDL). The labeling procedure had minimal effect on LDL size, morphology, or composition. Biological function was found to be maintained from both in vitro and in vivo experiments. Tumor-bearing mice were injected intravenously with LDL, DiR-LDL, Au-LDL, or a gold-loaded nanoemulsion. LDL accumulation in the tumors was detected with whole-body imaging methods, such as computed tomography (CT), spectral CT, and fluorescence imaging. Cellular localization was studied with transmission electron microscopy and fluorescence techniques. This LDL labeling procedure should permit the study of lipoprotein biointeractions in unprecedented detail

AB - Low-density lipoprotein (LDL) plays a critical role in cholesterol transport and is closely linked to the progression of several diseases. This motivates the development of methods to study LDL behavior from the microscopic to whole-body level. We have developed an approach to efficiently load LDL with a range of diagnostically active nanocrystals or hydrophobic agents. We performed focused experiments on LDL labeled with gold nanocrystals (Au-LDL). The labeling procedure had minimal effect on LDL size, morphology, or composition. Biological function was found to be maintained from both in vitro and in vivo experiments. Tumor-bearing mice were injected intravenously with LDL, DiR-LDL, Au-LDL, or a gold-loaded nanoemulsion. LDL accumulation in the tumors was detected with whole-body imaging methods, such as computed tomography (CT), spectral CT, and fluorescence imaging. Cellular localization was studied with transmission electron microscopy and fluorescence techniques. This LDL labeling procedure should permit the study of lipoprotein biointeractions in unprecedented detail

U2 - https://doi.org/10.1021/nn403258w

DO - https://doi.org/10.1021/nn403258w

M3 - Article

C2 - 24127782

SN - 1936-0851

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EP - 9770

JO - ACS nano

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IS - 11

ER -