Granzyme M and K release in human experimental endotoxemia

Annette C. Wensink; Maryse A. Wiewel; Lieneke H. Jongeneel; Marianne Boes; Tom van der Poll; C. Erik Hack; Niels Bovenschen

doi:https://doi.org/10.1016/j.imbio.2016.02.006

Granzyme M and K release in human experimental endotoxemia

Annette C. Wensink, Maryse A. Wiewel, Lieneke H. Jongeneel, Marianne Boes, Tom van der Poll, C. Erik Hack, Niels Bovenschen

Research output: Contribution to journal › Article › Academic › peer-review

12 Citations (Scopus)

Abstract

Granzymes are serine proteases involved in killing of tumor cells and virally infected cells. However, granzymes are also upregulated in blood under inflammatory conditions and contribute to cytokine release and processing. Here, we show that granzyme M (GrM) and to a lesser extent GrK are transiently elevated in the circulation following LPS administration in humans. GrM is released upon stimulation of whole blood with LPS or the gram-negative bacteria Escherichia coli BL21, Pseudomonas aeruginosa, and Neisseria meningitidis. GrK is only released upon stimulation with P. aeruginosa. Thus, GrM and GrK are differentially released in response to LPS and gram-negative bacteria

Original language	English
Pages (from-to)	773-777
Journal	Immunobiology
Volume	221
Issue number	7
DOIs	https://doi.org/10.1016/j.imbio.2016.02.006
Publication status	Published - 2016

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https://doi.org/10.1016/j.imbio.2016.02.006

Cite this

@article{0c678c445d124bd69dfffb8acdb86792,

title = "Granzyme M and K release in human experimental endotoxemia",

abstract = "Granzymes are serine proteases involved in killing of tumor cells and virally infected cells. However, granzymes are also upregulated in blood under inflammatory conditions and contribute to cytokine release and processing. Here, we show that granzyme M (GrM) and to a lesser extent GrK are transiently elevated in the circulation following LPS administration in humans. GrM is released upon stimulation of whole blood with LPS or the gram-negative bacteria Escherichia coli BL21, Pseudomonas aeruginosa, and Neisseria meningitidis. GrK is only released upon stimulation with P. aeruginosa. Thus, GrM and GrK are differentially released in response to LPS and gram-negative bacteria",

author = "Wensink, {Annette C.} and Wiewel, {Maryse A.} and Jongeneel, {Lieneke H.} and Marianne Boes and {van der Poll}, Tom and Hack, {C. Erik} and Niels Bovenschen",

year = "2016",

doi = "https://doi.org/10.1016/j.imbio.2016.02.006",

language = "English",

volume = "221",

pages = "773--777",

journal = "Immunobiology",

issn = "0171-2985",

publisher = "Urban und Fischer Verlag GmbH und Co. KG",

number = "7",

}

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T1 - Granzyme M and K release in human experimental endotoxemia

AU - Wensink, Annette C.

AU - Wiewel, Maryse A.

AU - Jongeneel, Lieneke H.

AU - Boes, Marianne

AU - van der Poll, Tom

AU - Hack, C. Erik

AU - Bovenschen, Niels

PY - 2016

Y1 - 2016

N2 - Granzymes are serine proteases involved in killing of tumor cells and virally infected cells. However, granzymes are also upregulated in blood under inflammatory conditions and contribute to cytokine release and processing. Here, we show that granzyme M (GrM) and to a lesser extent GrK are transiently elevated in the circulation following LPS administration in humans. GrM is released upon stimulation of whole blood with LPS or the gram-negative bacteria Escherichia coli BL21, Pseudomonas aeruginosa, and Neisseria meningitidis. GrK is only released upon stimulation with P. aeruginosa. Thus, GrM and GrK are differentially released in response to LPS and gram-negative bacteria

AB - Granzymes are serine proteases involved in killing of tumor cells and virally infected cells. However, granzymes are also upregulated in blood under inflammatory conditions and contribute to cytokine release and processing. Here, we show that granzyme M (GrM) and to a lesser extent GrK are transiently elevated in the circulation following LPS administration in humans. GrM is released upon stimulation of whole blood with LPS or the gram-negative bacteria Escherichia coli BL21, Pseudomonas aeruginosa, and Neisseria meningitidis. GrK is only released upon stimulation with P. aeruginosa. Thus, GrM and GrK are differentially released in response to LPS and gram-negative bacteria

U2 - https://doi.org/10.1016/j.imbio.2016.02.006

DO - https://doi.org/10.1016/j.imbio.2016.02.006

M3 - Article

C2 - 26928607

SN - 0171-2985

VL - 221

SP - 773

EP - 777

JO - Immunobiology

JF - Immunobiology

IS - 7

ER -