TY - JOUR
T1 - Herbal medicine IMOD suppresses LPS-induced production of proinflammatory cytokines in human dendritic cells
AU - Mirzaee, Saeedeh
AU - Drewniak, Agata
AU - Sarrami-Forooshani, Ramin
AU - Kaptein, Tanja M.
AU - Gharibdoost, Farhad
AU - Geijtenbeek, Teunis B. H.
PY - 2015
Y1 - 2015
N2 - Traditional medicines that stimulate or modulate the immune system can be used as innovative approaches to treat immunological diseases. The herbal medicine IMOD has been shown to strongly modulate immune responses in several animal studies as well as in clinical trials. However, little is known about the mechanisms of IMOD to modulate immunity. Here we have investigated whether IMOD modulates the immunological function of human dendritic cells (DCs). IMOD alone did not induce DC maturation nor production of cytokines. Notably, IMOD decreased the production of pro inflammatory cytokines IL-6, IL-12 p70, and TNF alpha by LPS-activated DCs at both mRNA and protein levels in a dose dependent manner. In contrast, treatment with IMOD did not affect LPS induced production of the anti-inflammatory cytokine IL-10. Furthermore, IMOD inhibited T cell activation/proliferation by LPS-treated DCs and skewed T-cells responses toward the T helper type 2 polarization. These data strongly indicate that IMOD has a potent immunomodulatory ability that affects TLR signaling and thereby modulates DC function. Insight into the immunomodulatory effect of herbal medicine IMOD may provide innovative strategies to affect the immune system and to help combat various diseases
AB - Traditional medicines that stimulate or modulate the immune system can be used as innovative approaches to treat immunological diseases. The herbal medicine IMOD has been shown to strongly modulate immune responses in several animal studies as well as in clinical trials. However, little is known about the mechanisms of IMOD to modulate immunity. Here we have investigated whether IMOD modulates the immunological function of human dendritic cells (DCs). IMOD alone did not induce DC maturation nor production of cytokines. Notably, IMOD decreased the production of pro inflammatory cytokines IL-6, IL-12 p70, and TNF alpha by LPS-activated DCs at both mRNA and protein levels in a dose dependent manner. In contrast, treatment with IMOD did not affect LPS induced production of the anti-inflammatory cytokine IL-10. Furthermore, IMOD inhibited T cell activation/proliferation by LPS-treated DCs and skewed T-cells responses toward the T helper type 2 polarization. These data strongly indicate that IMOD has a potent immunomodulatory ability that affects TLR signaling and thereby modulates DC function. Insight into the immunomodulatory effect of herbal medicine IMOD may provide innovative strategies to affect the immune system and to help combat various diseases
U2 - https://doi.org/10.3389/fphar.2015.00064
DO - https://doi.org/10.3389/fphar.2015.00064
M3 - Article
C2 - 25870561
SN - 1663-9812
VL - 6
SP - 64
JO - Frontiers in pharmacology
JF - Frontiers in pharmacology
ER -