HLA-G expression in trophoblast cells circulating in maternal peripheral blood during early pregnancy

I J van Wijk; S Griffioen; M L Tjoa; M A Mulders; J M van Vugt; Y W Loke; C B Oudejans

doi:https://doi.org/10.1067/mob.2001.112973

HLA-G expression in trophoblast cells circulating in maternal peripheral blood during early pregnancy

I J van Wijk, S Griffioen, M L Tjoa, M A Mulders, J M van Vugt, Y W Loke, C B Oudejans

Research output: Contribution to journal › Article › Academic › peer-review

49 Citations (Scopus)

Abstract

OBJECTIVE: The aim of this study was to assess the use of circulating trophoblast cells in maternal peripheral blood for noninvasive prenatal diagnosis of numeric chromosomal aberrations.

STUDY DESIGN: A combined procedure for immunocytochemical identification and deoxyribonucleic acid fluorescence in situ hybridization was used after a single enrichment step consisting of density gradient centrifugation. A specific HLA-G monoclonal antibody was used in combination with X and Y chromosome specific probes in deoxyribonucleic acid fluorescence in situ hybridization to confirm fetal identity of cells bearing HLA-G in the case of a male fetus.

RESULTS: We detected fetal trophoblast cells expressing HLA-G in maternal blood starting at 9 weeks' gestation. In addition to fetal sex prediction with X and Y chromosome-specific probes, fetal aneuploidy was confirmed in peripheral blood from a pregnancy complicated by trisomy 21.

CONCLUSION: Although the numbers of fetal cells were extremely low, the proof of concept was demonstrated. Early noninvasive prenatal screening for numeric chromosomal abnormalities with fetal trophoblast cells is feasible.

Original language	English
Pages (from-to)	991-7
Number of pages	7
Journal	American Journal of Obstetrics and Gynecology
Volume	184
Issue number	5
DOIs	https://doi.org/10.1067/mob.2001.112973
Publication status	Published - Apr 2001

Keywords

Antibodies, Monoclonal
Centrifugation, Density Gradient
Chromosome Aberrations
Female
HLA Antigens/biosynthesis
HLA-G Antigens
Histocompatibility Antigens Class I/biosynthesis
Humans
Immunohistochemistry
Immunophenotyping
In Situ Hybridization, Fluorescence
Pregnancy
Pregnancy Trimester, First/immunology
Prenatal Diagnosis/methods
Sex Determination Analysis
Trophoblasts/cytology

Access to Document

https://doi.org/10.1067/mob.2001.112973

Cite this

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title = "HLA-G expression in trophoblast cells circulating in maternal peripheral blood during early pregnancy",

abstract = "OBJECTIVE: The aim of this study was to assess the use of circulating trophoblast cells in maternal peripheral blood for noninvasive prenatal diagnosis of numeric chromosomal aberrations.STUDY DESIGN: A combined procedure for immunocytochemical identification and deoxyribonucleic acid fluorescence in situ hybridization was used after a single enrichment step consisting of density gradient centrifugation. A specific HLA-G monoclonal antibody was used in combination with X and Y chromosome specific probes in deoxyribonucleic acid fluorescence in situ hybridization to confirm fetal identity of cells bearing HLA-G in the case of a male fetus.RESULTS: We detected fetal trophoblast cells expressing HLA-G in maternal blood starting at 9 weeks' gestation. In addition to fetal sex prediction with X and Y chromosome-specific probes, fetal aneuploidy was confirmed in peripheral blood from a pregnancy complicated by trisomy 21.CONCLUSION: Although the numbers of fetal cells were extremely low, the proof of concept was demonstrated. Early noninvasive prenatal screening for numeric chromosomal abnormalities with fetal trophoblast cells is feasible.",

keywords = "Antibodies, Monoclonal, Centrifugation, Density Gradient, Chromosome Aberrations, Female, HLA Antigens/biosynthesis, HLA-G Antigens, Histocompatibility Antigens Class I/biosynthesis, Humans, Immunohistochemistry, Immunophenotyping, In Situ Hybridization, Fluorescence, Pregnancy, Pregnancy Trimester, First/immunology, Prenatal Diagnosis/methods, Sex Determination Analysis, Trophoblasts/cytology",

author = "{van Wijk}, {I J} and S Griffioen and Tjoa, {M L} and Mulders, {M A} and {van Vugt}, {J M} and Loke, {Y W} and Oudejans, {C B}",

year = "2001",

month = apr,

doi = "https://doi.org/10.1067/mob.2001.112973",

language = "English",

volume = "184",

pages = "991--7",

journal = "American Journal of Obstetrics and Gynecology",

issn = "0002-9378",

publisher = "Mosby Inc.",

number = "5",

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T1 - HLA-G expression in trophoblast cells circulating in maternal peripheral blood during early pregnancy

AU - van Wijk, I J

AU - Griffioen, S

AU - Tjoa, M L

AU - Mulders, M A

AU - van Vugt, J M

AU - Loke, Y W

AU - Oudejans, C B

PY - 2001/4

Y1 - 2001/4

N2 - OBJECTIVE: The aim of this study was to assess the use of circulating trophoblast cells in maternal peripheral blood for noninvasive prenatal diagnosis of numeric chromosomal aberrations.STUDY DESIGN: A combined procedure for immunocytochemical identification and deoxyribonucleic acid fluorescence in situ hybridization was used after a single enrichment step consisting of density gradient centrifugation. A specific HLA-G monoclonal antibody was used in combination with X and Y chromosome specific probes in deoxyribonucleic acid fluorescence in situ hybridization to confirm fetal identity of cells bearing HLA-G in the case of a male fetus.RESULTS: We detected fetal trophoblast cells expressing HLA-G in maternal blood starting at 9 weeks' gestation. In addition to fetal sex prediction with X and Y chromosome-specific probes, fetal aneuploidy was confirmed in peripheral blood from a pregnancy complicated by trisomy 21.CONCLUSION: Although the numbers of fetal cells were extremely low, the proof of concept was demonstrated. Early noninvasive prenatal screening for numeric chromosomal abnormalities with fetal trophoblast cells is feasible.

AB - OBJECTIVE: The aim of this study was to assess the use of circulating trophoblast cells in maternal peripheral blood for noninvasive prenatal diagnosis of numeric chromosomal aberrations.STUDY DESIGN: A combined procedure for immunocytochemical identification and deoxyribonucleic acid fluorescence in situ hybridization was used after a single enrichment step consisting of density gradient centrifugation. A specific HLA-G monoclonal antibody was used in combination with X and Y chromosome specific probes in deoxyribonucleic acid fluorescence in situ hybridization to confirm fetal identity of cells bearing HLA-G in the case of a male fetus.RESULTS: We detected fetal trophoblast cells expressing HLA-G in maternal blood starting at 9 weeks' gestation. In addition to fetal sex prediction with X and Y chromosome-specific probes, fetal aneuploidy was confirmed in peripheral blood from a pregnancy complicated by trisomy 21.CONCLUSION: Although the numbers of fetal cells were extremely low, the proof of concept was demonstrated. Early noninvasive prenatal screening for numeric chromosomal abnormalities with fetal trophoblast cells is feasible.

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KW - Histocompatibility Antigens Class I/biosynthesis

KW - Humans

KW - Immunohistochemistry

KW - Immunophenotyping

KW - In Situ Hybridization, Fluorescence

KW - Pregnancy

KW - Pregnancy Trimester, First/immunology

KW - Prenatal Diagnosis/methods

KW - Sex Determination Analysis

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U2 - https://doi.org/10.1067/mob.2001.112973

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