Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures

Michel de Vries; Krzysztof Pyrc; Ron Berkhout; Wilma Vermeulen-Oost; Ronald Dijkman; Maarten F. Jebbink; Sylvia Bruisten; Ben Berkhout; Lia van der Hoek

doi:https://doi.org/10.1128/JCM.02009-07

Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures

Michel de Vries, Krzysztof Pyrc, Ron Berkhout, Wilma Vermeulen-Oost, Ronald Dijkman, Maarten F. Jebbink, Sylvia Bruisten, Ben Berkhout, Lia van der Hoek

Research output: Contribution to journal › Article › Academic › peer-review

44 Citations (Scopus)

Abstract

Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR

Original language	English
Pages (from-to)	759-762
Journal	Journal of clinical microbiology
Volume	46
Issue number	2
DOIs	https://doi.org/10.1128/JCM.02009-07
Publication status	Published - 2008

Access to Document

https://doi.org/10.1128/JCM.02009-07

Cite this

@article{e95a94b054f34f5aa2c5a92b46277af9,

title = "Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures",

abstract = "Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR",

author = "{de Vries}, Michel and Krzysztof Pyrc and Ron Berkhout and Wilma Vermeulen-Oost and Ronald Dijkman and Jebbink, {Maarten F.} and Sylvia Bruisten and Ben Berkhout and {van der Hoek}, Lia",

year = "2008",

doi = "https://doi.org/10.1128/JCM.02009-07",

language = "English",

volume = "46",

pages = "759--762",

journal = "Journal of clinical microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "2",

}

TY - JOUR

T1 - Human parechovirus type 1, 3, 4, 5, and 6 detection in picornavirus cultures

AU - de Vries, Michel

AU - Pyrc, Krzysztof

AU - Berkhout, Ron

AU - Vermeulen-Oost, Wilma

AU - Dijkman, Ronald

AU - Jebbink, Maarten F.

AU - Bruisten, Sylvia

AU - Berkhout, Ben

AU - van der Hoek, Lia

PY - 2008

Y1 - 2008

N2 - Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR

AB - Picornavirus cultures that could not be typed in neutralization assays were analyzed by VP1 reverse transcription-PCR (RT-PCR) and a virus discovery tool (VIDISCA). Human parechoviruses (HPeVs) were frequently identified, among which were the uncommon isolates HPeV-4, HPeV-5, and HPeV-6. The HPeV-5 isolate could be amplified only by VIDISCA and not by VP1 RT-PCR

U2 - https://doi.org/10.1128/JCM.02009-07

DO - https://doi.org/10.1128/JCM.02009-07

M3 - Article

C2 - 18077635

SN - 0095-1137

VL - 46

SP - 759

EP - 762

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 2

ER -