Identification of a cDNA encoding an isoform of human CTP synthetase

A. B. van Kuilenburg; R. Meinsma; P. Vreken; H. R. Waterham; A. H. van Gennip

doi:https://doi.org/10.1016/S0167-4781(00)00141-X

Identification of a cDNA encoding an isoform of human CTP synthetase

A. B. van Kuilenburg, R. Meinsma, P. Vreken, H. R. Waterham, A. H. van Gennip

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Abstract

A full-length cDNA clone encoding an isoform of human CTP synthetase (type II) was isolated. A 1761-nucleotide open reading frame which corresponds to a protein of 586 amino acids with a predicted molecular mass of 65678 Da was identified. The predicted protein sequence showed 74% identity with the translation product of a previously identified human CTP synthetase cDNA clone (type I). The function of the human cDNA encoding type II CTP synthetase was verified by successful complementation of the cytidine-requiring CTP synthetase deficient mutant JF618 of Escherichia coli. The gene encoding type II CTP synthetase has been localized on chromosome Xp22

Original language	English
Pages (from-to)	548-552
Journal	BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
Volume	1492
Issue number	2-3
DOIs	https://doi.org/10.1016/S0167-4781(00)00141-X
Publication status	Published - 2000

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https://doi.org/10.1016/S0167-4781(00)00141-X

Cite this

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title = "Identification of a cDNA encoding an isoform of human CTP synthetase",

abstract = "A full-length cDNA clone encoding an isoform of human CTP synthetase (type II) was isolated. A 1761-nucleotide open reading frame which corresponds to a protein of 586 amino acids with a predicted molecular mass of 65678 Da was identified. The predicted protein sequence showed 74% identity with the translation product of a previously identified human CTP synthetase cDNA clone (type I). The function of the human cDNA encoding type II CTP synthetase was verified by successful complementation of the cytidine-requiring CTP synthetase deficient mutant JF618 of Escherichia coli. The gene encoding type II CTP synthetase has been localized on chromosome Xp22",

author = "{van Kuilenburg}, {A. B.} and R. Meinsma and P. Vreken and Waterham, {H. R.} and {van Gennip}, {A. H.}",

year = "2000",

doi = "https://doi.org/10.1016/S0167-4781(00)00141-X",

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T1 - Identification of a cDNA encoding an isoform of human CTP synthetase

AU - van Kuilenburg, A. B.

AU - Meinsma, R.

AU - Vreken, P.

AU - Waterham, H. R.

AU - van Gennip, A. H.

PY - 2000

Y1 - 2000

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AB - A full-length cDNA clone encoding an isoform of human CTP synthetase (type II) was isolated. A 1761-nucleotide open reading frame which corresponds to a protein of 586 amino acids with a predicted molecular mass of 65678 Da was identified. The predicted protein sequence showed 74% identity with the translation product of a previously identified human CTP synthetase cDNA clone (type I). The function of the human cDNA encoding type II CTP synthetase was verified by successful complementation of the cytidine-requiring CTP synthetase deficient mutant JF618 of Escherichia coli. The gene encoding type II CTP synthetase has been localized on chromosome Xp22

U2 - https://doi.org/10.1016/S0167-4781(00)00141-X

DO - https://doi.org/10.1016/S0167-4781(00)00141-X

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JO - BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION

JF - BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION

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