TY - JOUR
T1 - Identification of burkholderia Thailandensis with novel genotypes in the soil of central Sierra Leone
AU - Birnie, Emma
AU - van’t Hof, Senne
AU - Bijnsdorp, Anne
AU - Mansaray, Yembeh
AU - Huizenga, Erdi
AU - van der Ende, Arie
AU - Hugenholtz, Floor
AU - Grobusch, Martin P.
AU - Joost Wiersinga, W.
PY - 2019
Y1 - 2019
N2 - Background The soil-dwelling bacillus Burkholderia pseudomallei is the etiological-agent of the neglected and life-threatening emerging infection melioidosis. The distribution of B. pseudomallei in West Africa is unknown. In the present study we aimed to determine whether B. pseudomallei and B. thailandensis are present in the environment of central Sierra Leone. Methodology/Principal findings In June-July 2017, we conducted an environmental surveillance study–designed in accordance with existing consensus guidelines—in central Sierra Leone. A total of 1,000 soil samples (100 per site) were collected and cultured. B. pseudomallei was not identified in the soil, but we identified seven novel B. thailandensis sequence types with multi-locus sequence typing (MLST) and 16S rRNA gene sequence analyses. Conclusions/Significance The presence of B. pseudomallei was not demonstrated, however, multiple novel B. thailandensis sequence types were identified. More environmental and sequencing studies are needed to further understand the genetic diversity, evolution and virulence of these emerging organisms.
AB - Background The soil-dwelling bacillus Burkholderia pseudomallei is the etiological-agent of the neglected and life-threatening emerging infection melioidosis. The distribution of B. pseudomallei in West Africa is unknown. In the present study we aimed to determine whether B. pseudomallei and B. thailandensis are present in the environment of central Sierra Leone. Methodology/Principal findings In June-July 2017, we conducted an environmental surveillance study–designed in accordance with existing consensus guidelines—in central Sierra Leone. A total of 1,000 soil samples (100 per site) were collected and cultured. B. pseudomallei was not identified in the soil, but we identified seven novel B. thailandensis sequence types with multi-locus sequence typing (MLST) and 16S rRNA gene sequence analyses. Conclusions/Significance The presence of B. pseudomallei was not demonstrated, however, multiple novel B. thailandensis sequence types were identified. More environmental and sequencing studies are needed to further understand the genetic diversity, evolution and virulence of these emerging organisms.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85069799046&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/31199807
U2 - https://doi.org/10.1371/journal.pntd.0007402
DO - https://doi.org/10.1371/journal.pntd.0007402
M3 - Article
C2 - 31199807
SN - 1935-2727
VL - 13
JO - PLoS neglected tropical diseases
JF - PLoS neglected tropical diseases
IS - 6
M1 - e0007402
ER -