Identification of cell lines permissive for human coronavirus NL63

Oliver Schildgen; Maarten F. Jebbink; Michel de Vries; Krzysztov Pyrc; Ronald Dijkman; Arne Simon; Andreas Müller; Bernd Kupfer; Lia van der Hoek

doi:https://doi.org/10.1016/j.jviromet.2006.07.023

Identification of cell lines permissive for human coronavirus NL63

Oliver Schildgen, Maarten F. Jebbink, Michel de Vries, Krzysztov Pyrc, Ronald Dijkman, Arne Simon, Andreas Müller, Bernd Kupfer, Lia van der Hoek

Research output: Contribution to journal › Article › Academic › peer-review

39 Citations (Scopus)

Abstract

Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin-Darby-Canine-kidney cells and in an undefined monkey kidney cell line some replication was observed but massive exponential rise in virus yield lacked The results will lead to an improved routine diagnostic algorithm for the detection of the human coronavirus NL63

Original language	English
Pages (from-to)	207-210
Journal	Journal of virological methods
Volume	138
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2006.07.023
Publication status	Published - 2006

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https://doi.org/10.1016/j.jviromet.2006.07.023

Cite this

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title = "Identification of cell lines permissive for human coronavirus NL63",

abstract = "Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin-Darby-Canine-kidney cells and in an undefined monkey kidney cell line some replication was observed but massive exponential rise in virus yield lacked The results will lead to an improved routine diagnostic algorithm for the detection of the human coronavirus NL63",

author = "Oliver Schildgen and Jebbink, {Maarten F.} and {de Vries}, Michel and Krzysztov Pyrc and Ronald Dijkman and Arne Simon and Andreas M{\"u}ller and Bernd Kupfer and {van der Hoek}, Lia",

year = "2006",

doi = "https://doi.org/10.1016/j.jviromet.2006.07.023",

language = "English",

volume = "138",

pages = "207--210",

journal = "Journal of virological methods",

issn = "0166-0934",

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T1 - Identification of cell lines permissive for human coronavirus NL63

AU - Schildgen, Oliver

AU - Jebbink, Maarten F.

AU - de Vries, Michel

AU - Pyrc, Krzysztov

AU - Dijkman, Ronald

AU - Simon, Arne

AU - Müller, Andreas

AU - Kupfer, Bernd

AU - van der Hoek, Lia

PY - 2006

Y1 - 2006

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AB - Six cell lines routinely used in laboratories were tested for permissiveness to the infection with the newly identified human coronavirus NL63. Two monkey epithelial cell lines, LLC-MK2 and Vero-B4, showed a cytopathic effect (CPE) and clear viral replication, whereas no CPE or replication was observed in human lung fibroblasts MRC-5s. In Rhabdomyosarcoma cells, Madin-Darby-Canine-kidney cells and in an undefined monkey kidney cell line some replication was observed but massive exponential rise in virus yield lacked The results will lead to an improved routine diagnostic algorithm for the detection of the human coronavirus NL63

U2 - https://doi.org/10.1016/j.jviromet.2006.07.023

DO - https://doi.org/10.1016/j.jviromet.2006.07.023

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SN - 0166-0934

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SP - 207

EP - 210

JO - Journal of virological methods

JF - Journal of virological methods

IS - 1-2

ER -