Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses

T. B. Geijtenbeek; R. Torensma; S. J. van Vliet; G. C. van Duijnhoven; G. J. Adema; Y. van Kooyk; C. G. Figdor

doi:https://doi.org/10.1016/S0092-8674(00)80693-5

Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses

T. B. Geijtenbeek, R. Torensma, S. J. van Vliet, G. C. van Duijnhoven, G. J. Adema, Y. van Kooyk, C. G. Figdor

Research output: Contribution to journal › Article › Academic › peer-review

1496 Citations (Scopus)

Abstract

Contact between dendritic cells (DC) and resting T cells is essential to initiate a primary immune response. Here, we demonstrate that ICAM-3 expressed by resting T cells is important in this first contact with DC. We discovered that instead of the common ICAM-3 receptors LFA-1 and alphaDbeta2, a novel DC-specific C-type lectin, DC-SIGN, binds ICAM-3 with high affinity. DC-SIGN, which is abundantly expressed by DC both in vitro and in vivo, mediates transient adhesion with T cells. Since antibodies against DC-SIGN inhibit DC-induced proliferation of resting T cells, our findings predict that DC-SIGN enables T cell receptor engagement by stabilization of the DC-T cell contact zone

Original language	English
Pages (from-to)	575-585
Number of pages	11
Journal	Cell
Volume	100
Issue number	5
DOIs	https://doi.org/10.1016/S0092-8674(00)80693-5
Publication status	Published - 3 Mar 2000

Keywords

Animals
Antibodies, Monoclonal/pharmacology
Antigen Presentation
Antigens, CD
Antigens, Differentiation
Antigens/metabolism
Calcium/physiology
Cell Adhesion
Cell Adhesion Molecules/immunology
Cell Communication
Cells, Cultured
Dendritic Cells/immunology
Flow Cytometry
Gene Expression
Humans
Intercellular Adhesion Molecule-1/physiology
K562 Cells
Lectins, C-Type
Lectins/immunology
Lymphocyte Activation/physiology
Lymphocyte Function-Associated Antigen-1/physiology
Mannans/pharmacology
Mannose/metabolism
Mice
Mice, Inbred BALB C
Models, Immunological
Molecular Weight
Receptors, Cell Surface/immunology
Receptors, HIV/isolation & purification
Recombinant Fusion Proteins/physiology
T-Lymphocytes/cytology
Transfection

Access to Document

https://doi.org/10.1016/S0092-8674(00)80693-5

Cite this

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title = "Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses",

abstract = "Contact between dendritic cells (DC) and resting T cells is essential to initiate a primary immune response. Here, we demonstrate that ICAM-3 expressed by resting T cells is important in this first contact with DC. We discovered that instead of the common ICAM-3 receptors LFA-1 and alphaDbeta2, a novel DC-specific C-type lectin, DC-SIGN, binds ICAM-3 with high affinity. DC-SIGN, which is abundantly expressed by DC both in vitro and in vivo, mediates transient adhesion with T cells. Since antibodies against DC-SIGN inhibit DC-induced proliferation of resting T cells, our findings predict that DC-SIGN enables T cell receptor engagement by stabilization of the DC-T cell contact zone",

keywords = "Animals, Antibodies, Monoclonal/pharmacology, Antigen Presentation, Antigens, CD, Antigens, Differentiation, Antigens/metabolism, Calcium/physiology, Cell Adhesion, Cell Adhesion Molecules/immunology, Cell Communication, Cells, Cultured, Dendritic Cells/immunology, Flow Cytometry, Gene Expression, Humans, Intercellular Adhesion Molecule-1/physiology, K562 Cells, Lectins, C-Type, Lectins/immunology, Lymphocyte Activation/physiology, Lymphocyte Function-Associated Antigen-1/physiology, Mannans/pharmacology, Mannose/metabolism, Mice, Mice, Inbred BALB C, Models, Immunological, Molecular Weight, Receptors, Cell Surface/immunology, Receptors, HIV/isolation & purification, Recombinant Fusion Proteins/physiology, T-Lymphocytes/cytology, Transfection",

author = "Geijtenbeek, {T. B.} and R. Torensma and {van Vliet}, {S. J.} and {van Duijnhoven}, {G. C.} and Adema, {G. J.} and {van Kooyk}, Y. and Figdor, {C. G.}",

year = "2000",

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doi = "https://doi.org/10.1016/S0092-8674(00)80693-5",

language = "English",

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AU - Geijtenbeek, T. B.

AU - Torensma, R.

AU - van Vliet, S. J.

AU - van Duijnhoven, G. C.

AU - Adema, G. J.

AU - van Kooyk, Y.

AU - Figdor, C. G.

PY - 2000/3/3

Y1 - 2000/3/3

N2 - Contact between dendritic cells (DC) and resting T cells is essential to initiate a primary immune response. Here, we demonstrate that ICAM-3 expressed by resting T cells is important in this first contact with DC. We discovered that instead of the common ICAM-3 receptors LFA-1 and alphaDbeta2, a novel DC-specific C-type lectin, DC-SIGN, binds ICAM-3 with high affinity. DC-SIGN, which is abundantly expressed by DC both in vitro and in vivo, mediates transient adhesion with T cells. Since antibodies against DC-SIGN inhibit DC-induced proliferation of resting T cells, our findings predict that DC-SIGN enables T cell receptor engagement by stabilization of the DC-T cell contact zone

AB - Contact between dendritic cells (DC) and resting T cells is essential to initiate a primary immune response. Here, we demonstrate that ICAM-3 expressed by resting T cells is important in this first contact with DC. We discovered that instead of the common ICAM-3 receptors LFA-1 and alphaDbeta2, a novel DC-specific C-type lectin, DC-SIGN, binds ICAM-3 with high affinity. DC-SIGN, which is abundantly expressed by DC both in vitro and in vivo, mediates transient adhesion with T cells. Since antibodies against DC-SIGN inhibit DC-induced proliferation of resting T cells, our findings predict that DC-SIGN enables T cell receptor engagement by stabilization of the DC-T cell contact zone

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KW - Antigens, Differentiation

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KW - Cell Communication

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KW - Molecular Weight

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KW - Receptors, HIV/isolation & purification

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KW - T-Lymphocytes/cytology

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U2 - https://doi.org/10.1016/S0092-8674(00)80693-5

DO - https://doi.org/10.1016/S0092-8674(00)80693-5

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