Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses

T. B. Geijtenbeek, R. Torensma, S. J. van Vliet, G. C. van Duijnhoven, G. J. Adema, Y. van Kooyk, C. G. Figdor

Research output: Contribution to journalArticleAcademicpeer-review

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Contact between dendritic cells (DC) and resting T cells is essential to initiate a primary immune response. Here, we demonstrate that ICAM-3 expressed by resting T cells is important in this first contact with DC. We discovered that instead of the common ICAM-3 receptors LFA-1 and alphaDbeta2, a novel DC-specific C-type lectin, DC-SIGN, binds ICAM-3 with high affinity. DC-SIGN, which is abundantly expressed by DC both in vitro and in vivo, mediates transient adhesion with T cells. Since antibodies against DC-SIGN inhibit DC-induced proliferation of resting T cells, our findings predict that DC-SIGN enables T cell receptor engagement by stabilization of the DC-T cell contact zone
Original languageEnglish
Pages (from-to)575-585
Number of pages11
Issue number5
Publication statusPublished - 3 Mar 2000


  • Animals
  • Antibodies, Monoclonal/pharmacology
  • Antigen Presentation
  • Antigens, CD
  • Antigens, Differentiation
  • Antigens/metabolism
  • Calcium/physiology
  • Cell Adhesion
  • Cell Adhesion Molecules/immunology
  • Cell Communication
  • Cells, Cultured
  • Dendritic Cells/immunology
  • Flow Cytometry
  • Gene Expression
  • Humans
  • Intercellular Adhesion Molecule-1/physiology
  • K562 Cells
  • Lectins, C-Type
  • Lectins/immunology
  • Lymphocyte Activation/physiology
  • Lymphocyte Function-Associated Antigen-1/physiology
  • Mannans/pharmacology
  • Mannose/metabolism
  • Mice
  • Mice, Inbred BALB C
  • Models, Immunological
  • Molecular Weight
  • Receptors, Cell Surface/immunology
  • Receptors, HIV/isolation & purification
  • Recombinant Fusion Proteins/physiology
  • T-Lymphocytes/cytology
  • Transfection

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