Identification of genotypically diverse Cryptococcus neoformans and Cryptococcus gattii isolates by Luminex xMAP technology

M. Bovers; M. R. Diaz; F. Hagen; L. Spanjaard; B. Duim; C. E. Visser; H. L. Hoogveld; J. Scharringa; I. M. Hoepelman; J. W. Fell; T. Boekhout

doi:https://doi.org/10.1128/JCM.00223-07

Identification of genotypically diverse Cryptococcus neoformans and Cryptococcus gattii isolates by Luminex xMAP technology

M. Bovers, M. R. Diaz, F. Hagen, L. Spanjaard, B. Duim, C. E. Visser, H. L. Hoogveld, J. Scharringa, I. M. Hoepelman, J. W. Fell, T. Boekhout

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A Luminex suspension array, which had been developed for identification of Cryptococcus neoformans and Cryptococcus gattii isolates, was tested by genotyping a set of 58 mostly clinical isolates. All genotypes of C. neoformans and C. gattii were included. In addition, cerebrospinal fluid (CSF) obtained from patients with cryptococcal meningitis was used to investigate the feasibility of the technique for identification of the infecting strain. The suspension array correctly identified haploid isolates in all cases. Furthermore, hybrid isolates possessing two alleles of the Luminex probe region could be identified as hybrids. In CSF specimens, the genotype of the cryptococcal strains responsible for infection could be identified after optimization of the PCR conditions. However, further optimization of the DNA extraction protocol is needed to enhance the usability of the method in clinical practice

Original language	English
Pages (from-to)	1874-1883
Journal	Journal of clinical microbiology
Volume	45
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00223-07
Publication status	Published - 2007

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https://doi.org/10.1128/JCM.00223-07

Cite this

Bovers, M., Diaz, M. R., Hagen, F., Spanjaard, L., Duim, B., Visser, C. E., Hoogveld, H. L., Scharringa, J., Hoepelman, I. M., Fell, J. W., & Boekhout, T. (2007). Identification of genotypically diverse Cryptococcus neoformans and Cryptococcus gattii isolates by Luminex xMAP technology. Journal of clinical microbiology, 45(6), 1874-1883. https://doi.org/10.1128/JCM.00223-07

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abstract = "A Luminex suspension array, which had been developed for identification of Cryptococcus neoformans and Cryptococcus gattii isolates, was tested by genotyping a set of 58 mostly clinical isolates. All genotypes of C. neoformans and C. gattii were included. In addition, cerebrospinal fluid (CSF) obtained from patients with cryptococcal meningitis was used to investigate the feasibility of the technique for identification of the infecting strain. The suspension array correctly identified haploid isolates in all cases. Furthermore, hybrid isolates possessing two alleles of the Luminex probe region could be identified as hybrids. In CSF specimens, the genotype of the cryptococcal strains responsible for infection could be identified after optimization of the PCR conditions. However, further optimization of the DNA extraction protocol is needed to enhance the usability of the method in clinical practice",

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AU - Duim, B.

AU - Visser, C. E.

AU - Hoogveld, H. L.

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AU - Fell, J. W.

AU - Boekhout, T.

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