Abstract
BACKGROUND: Small, basic peanut proteins are often poorly extracted in pH-neutral buffers that are optimal for the extraction of peanut storage proteins such as Ara h 1. As a result, such proteins are easily missed as potential allergens.
OBJECTIVE: To analyse the allergenic composition of the basic peanut protein (BPP) fraction.
METHODS: A peanut extract prepared at pH 4 was fractionated by physicochemical procedures. Chemical analysis was performed by SDS-PAGE and mass spectrometry. Because immunoblotting was found to be inefficient for most of these small basic proteins, IgE-binding activity was measured by coupling the fractions to CNBr-activated Sepharose, followed by incubation with sera from 55 Dutch peanut-allergic children and 125 I-labelled anti-IgE.
RESULTS: Most IgE reactivity of the BPP fraction was due to the 5-7 kDa amino-terminal fragment of Ara h 1. This finding was confirmed by the use of the fragment in recombinant form, to which 25/55 of the sera was IgE-positive.
CONCLUSION: The amino-terminal fragment of Ara h 1, a member of a family of small anti-microbial proteins, is an allergen independent of the carboxy-terminal fragment of Ara h 1.
Original language | English |
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Pages (from-to) | 401-405 |
Number of pages | 5 |
Journal | Clinical and experimental allergy |
Volume | 50 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Mar 2020 |
Keywords
- IgE
- allergens and epitopes
- food allergy
- immunologic tests