Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus

H. D. Schallig; M. A. van Leeuwen; W. M. Hendrikx

doi:https://doi.org/10.1017/S0031182000076198

Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus

H. D. Schallig, M. A. van Leeuwen, W. M. Hendrikx

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Abstract

The excretory/secretory (E/S) products of adult Haemonchus contortus comprise of at least 15 polypeptides with molecular weights ranging from 10 to > 100 kDa. These E/S products induce an immune response in infected Texel sheep, as demonstrated by specific IgG1 levels and a significant lymphocyte proliferation index. Moreover, immunoblotting analysis revealed that sera of primary H. contortus-infected sheep specifically recognize a 24 kDa E/S product. In addition, sera of challenged sheep react strongly with a 15 kDa E/S product. The other E/S products of H. contortus showed immunoreactivity with serum samples of Haemonchus-infected sheep as well as with samples of sheep harbouring other trichostrongylid infections. These cross-reacting epitopes are the main cause of the lack of specificity of an E/S material-based ELISA. This ELISA can differentiate Haemonchus infections from Nematodirus battus infections, but not from Ostertagia circumcincta or Trichostrongylus colubriformis infections

Original language	English
Pages (from-to)	351-357
Journal	Parasitology
Volume	108 ( Pt 3)
DOIs	https://doi.org/10.1017/S0031182000076198
Publication status	Published - 1994

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https://doi.org/10.1017/S0031182000076198

Cite this

@article{5cbacf082c5e4008a031d982fcfa7cf6,

title = "Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus",

abstract = "The excretory/secretory (E/S) products of adult Haemonchus contortus comprise of at least 15 polypeptides with molecular weights ranging from 10 to > 100 kDa. These E/S products induce an immune response in infected Texel sheep, as demonstrated by specific IgG1 levels and a significant lymphocyte proliferation index. Moreover, immunoblotting analysis revealed that sera of primary H. contortus-infected sheep specifically recognize a 24 kDa E/S product. In addition, sera of challenged sheep react strongly with a 15 kDa E/S product. The other E/S products of H. contortus showed immunoreactivity with serum samples of Haemonchus-infected sheep as well as with samples of sheep harbouring other trichostrongylid infections. These cross-reacting epitopes are the main cause of the lack of specificity of an E/S material-based ELISA. This ELISA can differentiate Haemonchus infections from Nematodirus battus infections, but not from Ostertagia circumcincta or Trichostrongylus colubriformis infections",

author = "Schallig, {H. D.} and {van Leeuwen}, {M. A.} and Hendrikx, {W. M.}",

year = "1994",

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language = "English",

volume = "108 ( Pt 3)",

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journal = "Parasitology",

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T1 - Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus

AU - Schallig, H. D.

AU - van Leeuwen, M. A.

AU - Hendrikx, W. M.

PY - 1994

Y1 - 1994

N2 - The excretory/secretory (E/S) products of adult Haemonchus contortus comprise of at least 15 polypeptides with molecular weights ranging from 10 to > 100 kDa. These E/S products induce an immune response in infected Texel sheep, as demonstrated by specific IgG1 levels and a significant lymphocyte proliferation index. Moreover, immunoblotting analysis revealed that sera of primary H. contortus-infected sheep specifically recognize a 24 kDa E/S product. In addition, sera of challenged sheep react strongly with a 15 kDa E/S product. The other E/S products of H. contortus showed immunoreactivity with serum samples of Haemonchus-infected sheep as well as with samples of sheep harbouring other trichostrongylid infections. These cross-reacting epitopes are the main cause of the lack of specificity of an E/S material-based ELISA. This ELISA can differentiate Haemonchus infections from Nematodirus battus infections, but not from Ostertagia circumcincta or Trichostrongylus colubriformis infections

AB - The excretory/secretory (E/S) products of adult Haemonchus contortus comprise of at least 15 polypeptides with molecular weights ranging from 10 to > 100 kDa. These E/S products induce an immune response in infected Texel sheep, as demonstrated by specific IgG1 levels and a significant lymphocyte proliferation index. Moreover, immunoblotting analysis revealed that sera of primary H. contortus-infected sheep specifically recognize a 24 kDa E/S product. In addition, sera of challenged sheep react strongly with a 15 kDa E/S product. The other E/S products of H. contortus showed immunoreactivity with serum samples of Haemonchus-infected sheep as well as with samples of sheep harbouring other trichostrongylid infections. These cross-reacting epitopes are the main cause of the lack of specificity of an E/S material-based ELISA. This ELISA can differentiate Haemonchus infections from Nematodirus battus infections, but not from Ostertagia circumcincta or Trichostrongylus colubriformis infections

U2 - https://doi.org/10.1017/S0031182000076198

DO - https://doi.org/10.1017/S0031182000076198

M3 - Article

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VL - 108 ( Pt 3)

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JO - Parasitology

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