Immunocytochemical detection of prostaglandin E2 in microvasculature and in neurons of rat brain after administration of bacterial endotoxin

Anne Marie Van Dam; Madeleine Brouns; Winston Man-A-Hing; Frank Berkenbosch

doi:https://doi.org/10.1016/0006-8993(93)90922-A

Immunocytochemical detection of prostaglandin E₂ in microvasculature and in neurons of rat brain after administration of bacterial endotoxin

Anne Marie Van Dam, Madeleine Brouns, Winston Man-A-Hing, Frank Berkenbosch

Research output: Contribution to journal › Article › Academic › peer-review

92 Citations (Scopus)

Abstract

The aim of the present study was to identify the site of prostaglandin E₂ (PGE2) production in the brain in response to a pyrogenic dose of endotoxin. The presence of PGE2 was detected using immunocytochemistry on Bouin's fixed vibratome sections of control and endotoxin-treated rats. Peripheral administration of endotoxin caused a time-related stimulation of PGE2 immunoreactivity (irPGE2) in the choroid plexus and in the microvasculature of the brain. In addition to these sites, hypophysiotrophic neurons of the paraventricular nucleus (PVN) and supraopticus nucleus (SON) responded with induction of irPGE2 to endotoxin administration. Our data demonstrate that alterations in brain function in response to endotoxin may involve arachidonic metabolities such as PGE2 that are induced at the blood-brain barrier (microvasculature) and blood-liquour barrier (choroid plexus) and in hypohysiotrophic neurons of the rat brain.

Original language	English
Pages (from-to)	331-336
Number of pages	6
Journal	Brain Research
Volume	613
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(93)90922-A
Publication status	Published - 11 Jun 1993

Keywords

Animals
Brain microvasculature
Brain/drug effects
Cerebrovascular Circulation
Choroid plexus
Dinoprostone/analysis
Endotoxin
Endotoxins/toxicity
Immunohistochemistry/methods
Male
Microcirculation/drug effects
Neurons/drug effects
Organ Specificity
Paraventricular and supraoptic nucleus
Prostaglandin E
Rats
Rats, Wistar
Reference Values

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

https://doi.org/10.1016/0006-8993(93)90922-A

Cite this

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title = "Immunocytochemical detection of prostaglandin E2 in microvasculature and in neurons of rat brain after administration of bacterial endotoxin",

abstract = "The aim of the present study was to identify the site of prostaglandin E2 (PGE2) production in the brain in response to a pyrogenic dose of endotoxin. The presence of PGE2 was detected using immunocytochemistry on Bouin's fixed vibratome sections of control and endotoxin-treated rats. Peripheral administration of endotoxin caused a time-related stimulation of PGE2 immunoreactivity (irPGE2) in the choroid plexus and in the microvasculature of the brain. In addition to these sites, hypophysiotrophic neurons of the paraventricular nucleus (PVN) and supraopticus nucleus (SON) responded with induction of irPGE2 to endotoxin administration. Our data demonstrate that alterations in brain function in response to endotoxin may involve arachidonic metabolities such as PGE2 that are induced at the blood-brain barrier (microvasculature) and blood-liquour barrier (choroid plexus) and in hypohysiotrophic neurons of the rat brain.",

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author = "{Van Dam}, {Anne Marie} and Madeleine Brouns and Winston Man-A-Hing and Frank Berkenbosch",

year = "1993",

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T1 - Immunocytochemical detection of prostaglandin E2 in microvasculature and in neurons of rat brain after administration of bacterial endotoxin

AU - Van Dam, Anne Marie

AU - Brouns, Madeleine

AU - Man-A-Hing, Winston

AU - Berkenbosch, Frank

PY - 1993/6/11

Y1 - 1993/6/11

N2 - The aim of the present study was to identify the site of prostaglandin E2 (PGE2) production in the brain in response to a pyrogenic dose of endotoxin. The presence of PGE2 was detected using immunocytochemistry on Bouin's fixed vibratome sections of control and endotoxin-treated rats. Peripheral administration of endotoxin caused a time-related stimulation of PGE2 immunoreactivity (irPGE2) in the choroid plexus and in the microvasculature of the brain. In addition to these sites, hypophysiotrophic neurons of the paraventricular nucleus (PVN) and supraopticus nucleus (SON) responded with induction of irPGE2 to endotoxin administration. Our data demonstrate that alterations in brain function in response to endotoxin may involve arachidonic metabolities such as PGE2 that are induced at the blood-brain barrier (microvasculature) and blood-liquour barrier (choroid plexus) and in hypohysiotrophic neurons of the rat brain.

AB - The aim of the present study was to identify the site of prostaglandin E2 (PGE2) production in the brain in response to a pyrogenic dose of endotoxin. The presence of PGE2 was detected using immunocytochemistry on Bouin's fixed vibratome sections of control and endotoxin-treated rats. Peripheral administration of endotoxin caused a time-related stimulation of PGE2 immunoreactivity (irPGE2) in the choroid plexus and in the microvasculature of the brain. In addition to these sites, hypophysiotrophic neurons of the paraventricular nucleus (PVN) and supraopticus nucleus (SON) responded with induction of irPGE2 to endotoxin administration. Our data demonstrate that alterations in brain function in response to endotoxin may involve arachidonic metabolities such as PGE2 that are induced at the blood-brain barrier (microvasculature) and blood-liquour barrier (choroid plexus) and in hypohysiotrophic neurons of the rat brain.

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KW - Immunohistochemistry/methods

KW - Male

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KW - Neurons/drug effects

KW - Organ Specificity

KW - Paraventricular and supraoptic nucleus

KW - Prostaglandin E

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KW - Rats, Wistar

KW - Reference Values

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DO - https://doi.org/10.1016/0006-8993(93)90922-A

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