Immunocytochemical detection of prostaglandin E2 in microvasculature and in neurons of rat brain after administration of bacterial endotoxin

Anne Marie Van Dam, Madeleine Brouns, Winston Man-A-Hing, Frank Berkenbosch

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The aim of the present study was to identify the site of prostaglandin E2 (PGE2) production in the brain in response to a pyrogenic dose of endotoxin. The presence of PGE2 was detected using immunocytochemistry on Bouin's fixed vibratome sections of control and endotoxin-treated rats. Peripheral administration of endotoxin caused a time-related stimulation of PGE2 immunoreactivity (irPGE2) in the choroid plexus and in the microvasculature of the brain. In addition to these sites, hypophysiotrophic neurons of the paraventricular nucleus (PVN) and supraopticus nucleus (SON) responded with induction of irPGE2 to endotoxin administration. Our data demonstrate that alterations in brain function in response to endotoxin may involve arachidonic metabolities such as PGE2 that are induced at the blood-brain barrier (microvasculature) and blood-liquour barrier (choroid plexus) and in hypohysiotrophic neurons of the rat brain.

Original languageEnglish
Pages (from-to)331-336
Number of pages6
JournalBrain Research
Issue number2
Publication statusPublished - 11 Jun 1993


  • Animals
  • Brain microvasculature
  • Brain/drug effects
  • Cerebrovascular Circulation
  • Choroid plexus
  • Dinoprostone/analysis
  • Endotoxin
  • Endotoxins/toxicity
  • Immunohistochemistry/methods
  • Male
  • Microcirculation/drug effects
  • Neurons/drug effects
  • Organ Specificity
  • Paraventricular and supraoptic nucleus
  • Prostaglandin E
  • Rats
  • Rats, Wistar
  • Reference Values

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