Immunocytochemical evidence for the acidic nature of peroxisomes in methylotrophic yeasts

H. R. Waterham; I. Keizer-Gunnink; J. M. Goodman; W. Harder; M. Veenhuis

doi:https://doi.org/10.1016/0014-5793(90)80142-6

Immunocytochemical evidence for the acidic nature of peroxisomes in methylotrophic yeasts

H. R. Waterham, I. Keizer-Gunnink, J. M. Goodman, W. Harder, M. Veenhuis

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Abstract

The possible acidic nature of the peroxisomal matrix present in intact yeast cells was studied immunocytochemically, using the weak base DAMP as a probe. Spheroplasts of methanol-grown Candida boidinii and Hansenula polymorpha were regenerated and incubated with DAMP. After immunogold labelling, using antibodies against DAMP, a specific accumulation of gold particles was observed on the peroxisomal profiles. This labelling was absent in controls, performed in the presence of ionophores or chloroquine. These results support earlier observations, that in intact cells a pH-gradient exists across the peroxisomal membrane. Experiments, carried out on osmotically swollen spheroplasts indicated that maintenance of this pH-gradient is strongly related to the cell's integrity

Original language	English
Pages (from-to)	17-19
Journal	FEBS letters
Volume	262
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)80142-6
Publication status	Published - 1990

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https://doi.org/10.1016/0014-5793(90)80142-6

Cite this

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title = "Immunocytochemical evidence for the acidic nature of peroxisomes in methylotrophic yeasts",

abstract = "The possible acidic nature of the peroxisomal matrix present in intact yeast cells was studied immunocytochemically, using the weak base DAMP as a probe. Spheroplasts of methanol-grown Candida boidinii and Hansenula polymorpha were regenerated and incubated with DAMP. After immunogold labelling, using antibodies against DAMP, a specific accumulation of gold particles was observed on the peroxisomal profiles. This labelling was absent in controls, performed in the presence of ionophores or chloroquine. These results support earlier observations, that in intact cells a pH-gradient exists across the peroxisomal membrane. Experiments, carried out on osmotically swollen spheroplasts indicated that maintenance of this pH-gradient is strongly related to the cell's integrity",

author = "Waterham, {H. R.} and I. Keizer-Gunnink and Goodman, {J. M.} and W. Harder and M. Veenhuis",

year = "1990",

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T1 - Immunocytochemical evidence for the acidic nature of peroxisomes in methylotrophic yeasts

AU - Waterham, H. R.

AU - Keizer-Gunnink, I.

AU - Goodman, J. M.

AU - Harder, W.

AU - Veenhuis, M.

PY - 1990

Y1 - 1990

N2 - The possible acidic nature of the peroxisomal matrix present in intact yeast cells was studied immunocytochemically, using the weak base DAMP as a probe. Spheroplasts of methanol-grown Candida boidinii and Hansenula polymorpha were regenerated and incubated with DAMP. After immunogold labelling, using antibodies against DAMP, a specific accumulation of gold particles was observed on the peroxisomal profiles. This labelling was absent in controls, performed in the presence of ionophores or chloroquine. These results support earlier observations, that in intact cells a pH-gradient exists across the peroxisomal membrane. Experiments, carried out on osmotically swollen spheroplasts indicated that maintenance of this pH-gradient is strongly related to the cell's integrity

AB - The possible acidic nature of the peroxisomal matrix present in intact yeast cells was studied immunocytochemically, using the weak base DAMP as a probe. Spheroplasts of methanol-grown Candida boidinii and Hansenula polymorpha were regenerated and incubated with DAMP. After immunogold labelling, using antibodies against DAMP, a specific accumulation of gold particles was observed on the peroxisomal profiles. This labelling was absent in controls, performed in the presence of ionophores or chloroquine. These results support earlier observations, that in intact cells a pH-gradient exists across the peroxisomal membrane. Experiments, carried out on osmotically swollen spheroplasts indicated that maintenance of this pH-gradient is strongly related to the cell's integrity

U2 - https://doi.org/10.1016/0014-5793(90)80142-6

DO - https://doi.org/10.1016/0014-5793(90)80142-6

M3 - Article

C2 - 2156731

SN - 0014-5793

VL - 262

SP - 17

EP - 19

JO - FEBS letters

JF - FEBS letters

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