Immunofluorescence detection of liver cell membrane autoantibodies using PLC/PRF/5 cells

R. W. de Koning; M. Daemen; B. Schutte; H. G. Geertzen; G. H. Blijham; F. T. Bosman

doi:https://doi.org/10.1111/j.1365-2362.1985.tb00146.x

Immunofluorescence detection of liver cell membrane autoantibodies using PLC/PRF/5 cells

R. W. de Koning, M. Daemen, B. Schutte, H. G. Geertzen, G. H. Blijham, F. T. Bosman

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Abstract

Detection of liver cell membrane autoantibodies is routinely performed by immunofluorescence testing of patient sera on rabbit hepatocyte suspensions. We have investigated the possible use of cells from the PLC/PRF/5 human hepatoma cell-line. These cells were employed as substrate in an immunofluorescence test which was compared with the conventional rabbit hepatocyte assay. We found a close correlation between the results obtained with these different substrates on visual reading. We furthermore compared visual reading of immunofluorescence preparations with flow-cytometrical analysis of immunostained cell suspensions. The results with these different methods were largely confirmatory. The PLC/PRF/5 cells are easily available and should therefore be regarded as a highly valuable new substrate for detection of liver cell membrane autoantibodies. Flow cytometry appears to be a technically simple and reliable method for quantitative analysis

Original language	English
Pages (from-to)	64-68
Journal	European journal of clinical investigation
Volume	15
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2362.1985.tb00146.x
Publication status	Published - 1985

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https://doi.org/10.1111/j.1365-2362.1985.tb00146.x

Cite this

@article{0845dabf048442cba51c0abbbf570ccb,

title = "Immunofluorescence detection of liver cell membrane autoantibodies using PLC/PRF/5 cells",

abstract = "Detection of liver cell membrane autoantibodies is routinely performed by immunofluorescence testing of patient sera on rabbit hepatocyte suspensions. We have investigated the possible use of cells from the PLC/PRF/5 human hepatoma cell-line. These cells were employed as substrate in an immunofluorescence test which was compared with the conventional rabbit hepatocyte assay. We found a close correlation between the results obtained with these different substrates on visual reading. We furthermore compared visual reading of immunofluorescence preparations with flow-cytometrical analysis of immunostained cell suspensions. The results with these different methods were largely confirmatory. The PLC/PRF/5 cells are easily available and should therefore be regarded as a highly valuable new substrate for detection of liver cell membrane autoantibodies. Flow cytometry appears to be a technically simple and reliable method for quantitative analysis",

author = "{de Koning}, {R. W.} and M. Daemen and B. Schutte and Geertzen, {H. G.} and Blijham, {G. H.} and Bosman, {F. T.}",

year = "1985",

doi = "https://doi.org/10.1111/j.1365-2362.1985.tb00146.x",

language = "English",

volume = "15",

pages = "64--68",

journal = "European journal of clinical investigation",

issn = "0014-2972",

publisher = "Wiley-Blackwell Publishing",

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TY - JOUR

T1 - Immunofluorescence detection of liver cell membrane autoantibodies using PLC/PRF/5 cells

AU - de Koning, R. W.

AU - Daemen, M.

AU - Schutte, B.

AU - Geertzen, H. G.

AU - Blijham, G. H.

AU - Bosman, F. T.

PY - 1985

Y1 - 1985

N2 - Detection of liver cell membrane autoantibodies is routinely performed by immunofluorescence testing of patient sera on rabbit hepatocyte suspensions. We have investigated the possible use of cells from the PLC/PRF/5 human hepatoma cell-line. These cells were employed as substrate in an immunofluorescence test which was compared with the conventional rabbit hepatocyte assay. We found a close correlation between the results obtained with these different substrates on visual reading. We furthermore compared visual reading of immunofluorescence preparations with flow-cytometrical analysis of immunostained cell suspensions. The results with these different methods were largely confirmatory. The PLC/PRF/5 cells are easily available and should therefore be regarded as a highly valuable new substrate for detection of liver cell membrane autoantibodies. Flow cytometry appears to be a technically simple and reliable method for quantitative analysis

AB - Detection of liver cell membrane autoantibodies is routinely performed by immunofluorescence testing of patient sera on rabbit hepatocyte suspensions. We have investigated the possible use of cells from the PLC/PRF/5 human hepatoma cell-line. These cells were employed as substrate in an immunofluorescence test which was compared with the conventional rabbit hepatocyte assay. We found a close correlation between the results obtained with these different substrates on visual reading. We furthermore compared visual reading of immunofluorescence preparations with flow-cytometrical analysis of immunostained cell suspensions. The results with these different methods were largely confirmatory. The PLC/PRF/5 cells are easily available and should therefore be regarded as a highly valuable new substrate for detection of liver cell membrane autoantibodies. Flow cytometry appears to be a technically simple and reliable method for quantitative analysis

U2 - https://doi.org/10.1111/j.1365-2362.1985.tb00146.x

DO - https://doi.org/10.1111/j.1365-2362.1985.tb00146.x

M3 - Article

C2 - 2986983

SN - 0014-2972

VL - 15

SP - 64

EP - 68

JO - European journal of clinical investigation

JF - European journal of clinical investigation

IS - 2

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