Immunohistochemical analysis of the internal limiting membrane peeled with infracyanine green

Ellen C La Heij; Suzanne C Dieudonné; Cornelia M Mooy; Roselie M H Diederen; Albert T A Liem; Robert J van Suylen; Fred Hendrikse

doi:https://doi.org/10.1016/j.ajo.2005.05.054

Immunohistochemical analysis of the internal limiting membrane peeled with infracyanine green

Ellen C La Heij, Suzanne C Dieudonné, Cornelia M Mooy, Roselie M H Diederen, Albert T A Liem, Robert J van Suylen, Fred Hendrikse

Ophthalmology (AMC)

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

PURPOSE: To investigate whether the peeled internal limiting membrane (ILM) contains cellular retinal cell fragments, and to learn more about their possible origin.

DESIGN: Experimental study.

METHODS: ILM peeled from ten eyes during vitrectomy by infracyanine green (ICG) was studied immunohistochemically using the markers: GFAP, S-100, and vimentin. Five ILM specimens were from eyes with diabetic macular edema (DME), two from eyes with a macular hole, and three from eyes with persisting macular edema after retinal detachment surgery.

RESULTS: In eight of the ten ILM specimens, we found GFAP-positive staining, indicating the presence of remnants of footplates from Müller cells or glial cells. Two ILM specimens were positive for S-100, indicating the presence of neural cells or ganglion cells.

CONCLUSIONS: ILM peeled from the retina during vitrectomy using ICG may contain remnants of Müller cell footplates, neural cells, and ganglion cells.

Original language	English
Pages (from-to)	1123-5
Number of pages	3
Journal	American Journal of Ophthalmology
Volume	140
Issue number	6
DOIs	https://doi.org/10.1016/j.ajo.2005.05.054
Publication status	Published - Dec 2005

Keywords

Aged
Basement Membrane/metabolism
Coloring Agents
Diabetic Retinopathy/surgery
Epiretinal Membrane/metabolism
Female
Glial Fibrillary Acidic Protein/metabolism
Humans
Immunoenzyme Techniques
Indocyanine Green/analogs & derivatives
Macular Edema/surgery
Male
Middle Aged
Neuroglia/pathology
Retinal Detachment/surgery
Retinal Perforations/surgery
S100 Proteins/metabolism
Vimentin/metabolism
Vitrectomy

Access to Document

https://doi.org/10.1016/j.ajo.2005.05.054

Cite this

@article{186bd281e1e34a8da7bd844b682b380b,

title = "Immunohistochemical analysis of the internal limiting membrane peeled with infracyanine green",

abstract = "PURPOSE: To investigate whether the peeled internal limiting membrane (ILM) contains cellular retinal cell fragments, and to learn more about their possible origin.DESIGN: Experimental study.METHODS: ILM peeled from ten eyes during vitrectomy by infracyanine green (ICG) was studied immunohistochemically using the markers: GFAP, S-100, and vimentin. Five ILM specimens were from eyes with diabetic macular edema (DME), two from eyes with a macular hole, and three from eyes with persisting macular edema after retinal detachment surgery.RESULTS: In eight of the ten ILM specimens, we found GFAP-positive staining, indicating the presence of remnants of footplates from M{\"u}ller cells or glial cells. Two ILM specimens were positive for S-100, indicating the presence of neural cells or ganglion cells.CONCLUSIONS: ILM peeled from the retina during vitrectomy using ICG may contain remnants of M{\"u}ller cell footplates, neural cells, and ganglion cells.",

keywords = "Aged, Basement Membrane/metabolism, Coloring Agents, Diabetic Retinopathy/surgery, Epiretinal Membrane/metabolism, Female, Glial Fibrillary Acidic Protein/metabolism, Humans, Immunoenzyme Techniques, Indocyanine Green/analogs & derivatives, Macular Edema/surgery, Male, Middle Aged, Neuroglia/pathology, Retinal Detachment/surgery, Retinal Perforations/surgery, S100 Proteins/metabolism, Vimentin/metabolism, Vitrectomy",

author = "{La Heij}, {Ellen C} and Dieudonn{\'e}, {Suzanne C} and Mooy, {Cornelia M} and Diederen, {Roselie M H} and Liem, {Albert T A} and {van Suylen}, {Robert J} and Fred Hendrikse",

year = "2005",

month = dec,

doi = "https://doi.org/10.1016/j.ajo.2005.05.054",

language = "English",

volume = "140",

pages = "1123--5",

journal = "American Journal of Ophthalmology",

issn = "0002-9394",

publisher = "Elsevier USA",

number = "6",

}

TY - JOUR

T1 - Immunohistochemical analysis of the internal limiting membrane peeled with infracyanine green

AU - La Heij, Ellen C

AU - Dieudonné, Suzanne C

AU - Mooy, Cornelia M

AU - Diederen, Roselie M H

AU - Liem, Albert T A

AU - van Suylen, Robert J

AU - Hendrikse, Fred

PY - 2005/12

Y1 - 2005/12

N2 - PURPOSE: To investigate whether the peeled internal limiting membrane (ILM) contains cellular retinal cell fragments, and to learn more about their possible origin.DESIGN: Experimental study.METHODS: ILM peeled from ten eyes during vitrectomy by infracyanine green (ICG) was studied immunohistochemically using the markers: GFAP, S-100, and vimentin. Five ILM specimens were from eyes with diabetic macular edema (DME), two from eyes with a macular hole, and three from eyes with persisting macular edema after retinal detachment surgery.RESULTS: In eight of the ten ILM specimens, we found GFAP-positive staining, indicating the presence of remnants of footplates from Müller cells or glial cells. Two ILM specimens were positive for S-100, indicating the presence of neural cells or ganglion cells.CONCLUSIONS: ILM peeled from the retina during vitrectomy using ICG may contain remnants of Müller cell footplates, neural cells, and ganglion cells.

AB - PURPOSE: To investigate whether the peeled internal limiting membrane (ILM) contains cellular retinal cell fragments, and to learn more about their possible origin.DESIGN: Experimental study.METHODS: ILM peeled from ten eyes during vitrectomy by infracyanine green (ICG) was studied immunohistochemically using the markers: GFAP, S-100, and vimentin. Five ILM specimens were from eyes with diabetic macular edema (DME), two from eyes with a macular hole, and three from eyes with persisting macular edema after retinal detachment surgery.RESULTS: In eight of the ten ILM specimens, we found GFAP-positive staining, indicating the presence of remnants of footplates from Müller cells or glial cells. Two ILM specimens were positive for S-100, indicating the presence of neural cells or ganglion cells.CONCLUSIONS: ILM peeled from the retina during vitrectomy using ICG may contain remnants of Müller cell footplates, neural cells, and ganglion cells.

KW - Aged

KW - Basement Membrane/metabolism

KW - Coloring Agents

KW - Diabetic Retinopathy/surgery

KW - Epiretinal Membrane/metabolism

KW - Female

KW - Glial Fibrillary Acidic Protein/metabolism

KW - Humans

KW - Immunoenzyme Techniques

KW - Indocyanine Green/analogs & derivatives

KW - Macular Edema/surgery

KW - Male

KW - Middle Aged

KW - Neuroglia/pathology

KW - Retinal Detachment/surgery

KW - Retinal Perforations/surgery

KW - S100 Proteins/metabolism

KW - Vimentin/metabolism

KW - Vitrectomy

U2 - https://doi.org/10.1016/j.ajo.2005.05.054

DO - https://doi.org/10.1016/j.ajo.2005.05.054

M3 - Article

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