Impedimetric DNA biosensor based on polyurethane/poly(m-anthranilic acid) nanofibers

Zeliha Guler Gokce; P. nar Akalın; Fatma Neşe Kok; A. Sezai Sarac

doi:https://doi.org/10.1016/j.snb.2017.07.136

Impedimetric DNA biosensor based on polyurethane/poly(m-anthranilic acid) nanofibers

Zeliha Guler Gokce, P. nar Akalın, Fatma Neşe Kok, A. Sezai Sarac

Research output: Contribution to journal › Article › Academic › peer-review

27 Citations (Scopus)

Abstract

An impedimetric DNA biosensor based on nanofiber mats were fabricated for the detection of Salmonella spp. Polyurethane/Poly(m-anthranilic acid) (PU/P3ANA) nanofibers were obtained by electrospinning. Capture DNA probe was covalently immobilized onto PU/P3ANA nanofibers by activation of carboxyl groups on the nanofibers. The hybridization was carried out with fully complementary and mutant target DNA with a single or double-base mismatch. The immobilization and hybridization process was investigated by electrochemical impedance spectroscopy (EIS) and the data were fitted to equivalent electrical circuit model (ECM). The hybridization of complementary DNA with the immobilized probe DNA increased the charge transfer resistance of the PU/P3ANA nanofibers. The fabricated DNA biosensor was stable up to one month and displayed linear response (0.1 μM − 10 μM), high selectivity (8.17 kΩ/μM) and single-base mismatch selectivity. This study represents a sensitive and advanced nanofiber based DNA biosensor for the detection of Salmonella spp. by EIS.

Original language	English
Pages (from-to)	719-726
Journal	Sensors and Actuators, B: Chemical
Volume	254
DOIs	https://doi.org/10.1016/j.snb.2017.07.136
Publication status	Published - 2018
Externally published	Yes

Access to Document

https://doi.org/10.1016/j.snb.2017.07.136

Cite this

@article{1efa76a6b9a24f38b2355cb2ee264639,

title = "Impedimetric DNA biosensor based on polyurethane/poly(m-anthranilic acid) nanofibers",

abstract = "An impedimetric DNA biosensor based on nanofiber mats were fabricated for the detection of Salmonella spp. Polyurethane/Poly(m-anthranilic acid) (PU/P3ANA) nanofibers were obtained by electrospinning. Capture DNA probe was covalently immobilized onto PU/P3ANA nanofibers by activation of carboxyl groups on the nanofibers. The hybridization was carried out with fully complementary and mutant target DNA with a single or double-base mismatch. The immobilization and hybridization process was investigated by electrochemical impedance spectroscopy (EIS) and the data were fitted to equivalent electrical circuit model (ECM). The hybridization of complementary DNA with the immobilized probe DNA increased the charge transfer resistance of the PU/P3ANA nanofibers. The fabricated DNA biosensor was stable up to one month and displayed linear response (0.1 μM − 10 μM), high selectivity (8.17 kΩ/μM) and single-base mismatch selectivity. This study represents a sensitive and advanced nanofiber based DNA biosensor for the detection of Salmonella spp. by EIS.",

author = "{Guler Gokce}, Zeliha and Akalın, {P. nar} and Kok, {Fatma Ne{\c s}e} and Sarac, {A. Sezai}",

year = "2018",

doi = "https://doi.org/10.1016/j.snb.2017.07.136",

language = "English",

volume = "254",

pages = "719--726",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier",

}

TY - JOUR

T1 - Impedimetric DNA biosensor based on polyurethane/poly(m-anthranilic acid) nanofibers

AU - Guler Gokce, Zeliha

AU - Akalın, P. nar

AU - Kok, Fatma Neşe

AU - Sarac, A. Sezai

PY - 2018

Y1 - 2018

N2 - An impedimetric DNA biosensor based on nanofiber mats were fabricated for the detection of Salmonella spp. Polyurethane/Poly(m-anthranilic acid) (PU/P3ANA) nanofibers were obtained by electrospinning. Capture DNA probe was covalently immobilized onto PU/P3ANA nanofibers by activation of carboxyl groups on the nanofibers. The hybridization was carried out with fully complementary and mutant target DNA with a single or double-base mismatch. The immobilization and hybridization process was investigated by electrochemical impedance spectroscopy (EIS) and the data were fitted to equivalent electrical circuit model (ECM). The hybridization of complementary DNA with the immobilized probe DNA increased the charge transfer resistance of the PU/P3ANA nanofibers. The fabricated DNA biosensor was stable up to one month and displayed linear response (0.1 μM − 10 μM), high selectivity (8.17 kΩ/μM) and single-base mismatch selectivity. This study represents a sensitive and advanced nanofiber based DNA biosensor for the detection of Salmonella spp. by EIS.

AB - An impedimetric DNA biosensor based on nanofiber mats were fabricated for the detection of Salmonella spp. Polyurethane/Poly(m-anthranilic acid) (PU/P3ANA) nanofibers were obtained by electrospinning. Capture DNA probe was covalently immobilized onto PU/P3ANA nanofibers by activation of carboxyl groups on the nanofibers. The hybridization was carried out with fully complementary and mutant target DNA with a single or double-base mismatch. The immobilization and hybridization process was investigated by electrochemical impedance spectroscopy (EIS) and the data were fitted to equivalent electrical circuit model (ECM). The hybridization of complementary DNA with the immobilized probe DNA increased the charge transfer resistance of the PU/P3ANA nanofibers. The fabricated DNA biosensor was stable up to one month and displayed linear response (0.1 μM − 10 μM), high selectivity (8.17 kΩ/μM) and single-base mismatch selectivity. This study represents a sensitive and advanced nanofiber based DNA biosensor for the detection of Salmonella spp. by EIS.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85026377697&origin=inward

U2 - https://doi.org/10.1016/j.snb.2017.07.136

DO - https://doi.org/10.1016/j.snb.2017.07.136

M3 - Article

SN - 0925-4005

VL - 254

SP - 719

EP - 726

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

Impedimetric DNA biosensor based on polyurethane/poly(m-anthranilic acid) nanofibers

Abstract

Access to Document

Other files and links

Cite this