Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR

R. Roosendaal; E. J. Kuipers; A. J.C. Van den Brule; A. Salvador Pena; A. M. Uyterlinde; J. M.M. Walboomers; S. G.M. Meuwissen; J. De Graaff

doi:https://doi.org/10.1128/jcm.32.4.1123-1126.1994

Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR

R. Roosendaal, E. J. Kuipers, A. J.C. Van den Brule, A. Salvador Pena, A. M. Uyterlinde, J. M.M. Walboomers, S. G.M. Meuwissen, J. De Graaff

Research output: Contribution to journal › Comment/Letter to the editor › Academic

53 Citations (Scopus)

Abstract

A 16S ribosomal DNA-based PCR appeared to be a sensitive test for the detection of infection by Helicobacter pylori in 31 patients when compared with culturing and histological and serological techniques. For five patients, PCR was the only test with a positive result. H. pylori DNA was also found in gastrointestinal equipment even after standard intensive combined manual and machine cleaning. We therefore conclude that a reliable validation of PCR for the detection of H. pylori in gastric biopsy specimens is possible only when the cleaning and disinfection method used has been proven to remove all H. pylori DNA from gastrointestinal equipment. An adequate cleaning and disinfection method for the removal of H. pylori DNA from fiberoptic endoscopes is described.

Original language	English
Pages (from-to)	1123-1126
Number of pages	4
Journal	Journal of clinical microbiology
Volume	32
Issue number	4
DOIs	https://doi.org/10.1128/jcm.32.4.1123-1126.1994
Publication status	Published - 1994

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Roosendaal, R., Kuipers, E. J., Van den Brule, A. J. C., Salvador Pena, A., Uyterlinde, A. M., Walboomers, J. M. M., Meuwissen, S. G. M., & De Graaff, J. (1994). Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR. Journal of clinical microbiology, 32(4), 1123-1126. https://doi.org/10.1128/jcm.32.4.1123-1126.1994

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title = "Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR",

abstract = "A 16S ribosomal DNA-based PCR appeared to be a sensitive test for the detection of infection by Helicobacter pylori in 31 patients when compared with culturing and histological and serological techniques. For five patients, PCR was the only test with a positive result. H. pylori DNA was also found in gastrointestinal equipment even after standard intensive combined manual and machine cleaning. We therefore conclude that a reliable validation of PCR for the detection of H. pylori in gastric biopsy specimens is possible only when the cleaning and disinfection method used has been proven to remove all H. pylori DNA from gastrointestinal equipment. An adequate cleaning and disinfection method for the removal of H. pylori DNA from fiberoptic endoscopes is described.",

author = "R. Roosendaal and Kuipers, {E. J.} and {Van den Brule}, {A. J.C.} and {Salvador Pena}, A. and Uyterlinde, {A. M.} and Walboomers, {J. M.M.} and Meuwissen, {S. G.M.} and {De Graaff}, J.",

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Roosendaal, R, Kuipers, EJ, Van den Brule, AJC, Salvador Pena, A , Uyterlinde, AM, Walboomers, JMM, Meuwissen, SGM & De Graaff, J 1994, 'Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR', Journal of clinical microbiology, vol. 32, no. 4, pp. 1123-1126. https://doi.org/10.1128/jcm.32.4.1123-1126.1994

TY - JOUR

T1 - Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR

AU - Roosendaal, R.

AU - Kuipers, E. J.

AU - Van den Brule, A. J.C.

AU - Salvador Pena, A.

AU - Uyterlinde, A. M.

AU - Walboomers, J. M.M.

AU - Meuwissen, S. G.M.

AU - De Graaff, J.

PY - 1994

Y1 - 1994

N2 - A 16S ribosomal DNA-based PCR appeared to be a sensitive test for the detection of infection by Helicobacter pylori in 31 patients when compared with culturing and histological and serological techniques. For five patients, PCR was the only test with a positive result. H. pylori DNA was also found in gastrointestinal equipment even after standard intensive combined manual and machine cleaning. We therefore conclude that a reliable validation of PCR for the detection of H. pylori in gastric biopsy specimens is possible only when the cleaning and disinfection method used has been proven to remove all H. pylori DNA from gastrointestinal equipment. An adequate cleaning and disinfection method for the removal of H. pylori DNA from fiberoptic endoscopes is described.

AB - A 16S ribosomal DNA-based PCR appeared to be a sensitive test for the detection of infection by Helicobacter pylori in 31 patients when compared with culturing and histological and serological techniques. For five patients, PCR was the only test with a positive result. H. pylori DNA was also found in gastrointestinal equipment even after standard intensive combined manual and machine cleaning. We therefore conclude that a reliable validation of PCR for the detection of H. pylori in gastric biopsy specimens is possible only when the cleaning and disinfection method used has been proven to remove all H. pylori DNA from gastrointestinal equipment. An adequate cleaning and disinfection method for the removal of H. pylori DNA from fiberoptic endoscopes is described.

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U2 - https://doi.org/10.1128/jcm.32.4.1123-1126.1994

DO - https://doi.org/10.1128/jcm.32.4.1123-1126.1994

M3 - Comment/Letter to the editor

C2 - 7517952

SN - 0095-1137

VL - 32

SP - 1123

EP - 1126

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 4

ER -

Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR

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