TY - JOUR
T1 - Improved method for the determination of serotonin in plasma by high-performance liquid chromatography using on-line sample pre-treatment
AU - Takkenberg, B.
AU - Endert, E.
AU - van Ingen, H. E.
AU - Ackermans, M.
PY - 1991
Y1 - 1991
N2 - An improved method for the determination of serotonin in platelet-rich plasma (PRP) and platelet-poor plasma (PPP), by reversed-phase high-performance liquid chromatography with electrochemical detection and direct plasma injection, is described. The chromatographic system comprises a strong cation-exchange pre-column and a C18 analytical column. The method is selective, rapid, simple and sensitive, and offers good reproducibility and recovery. Reference values for serotonin concentrations in healthy adults (n = 10) are 31 nM for PPP and 6 nmol per 10(9) platelets for PRP. The conditions used for the preparation of PRP and PPP may influence the serotonin concentration in PRP
AB - An improved method for the determination of serotonin in platelet-rich plasma (PRP) and platelet-poor plasma (PPP), by reversed-phase high-performance liquid chromatography with electrochemical detection and direct plasma injection, is described. The chromatographic system comprises a strong cation-exchange pre-column and a C18 analytical column. The method is selective, rapid, simple and sensitive, and offers good reproducibility and recovery. Reference values for serotonin concentrations in healthy adults (n = 10) are 31 nM for PPP and 6 nmol per 10(9) platelets for PRP. The conditions used for the preparation of PRP and PPP may influence the serotonin concentration in PRP
U2 - https://doi.org/10.1016/0378-4347(91)80405-2
DO - https://doi.org/10.1016/0378-4347(91)80405-2
M3 - Comment/Letter to the editor
C2 - 1874888
SN - 0021-9673
VL - 565
SP - 430
EP - 435
JO - Journal of chromatography. A
JF - Journal of chromatography. A
IS - 1-2
ER -