TY - JOUR
T1 - In-depth analysis of immunohistochemistry concordance in biopsy-resection pairs of bronchial carcinoids
AU - Naves, Dwayne D.
AU - Reuling, Ellen M. B. P.
AU - Dickhoff, Chris
AU - Kortman, Pim C.
AU - Broeckaert, Mark A. M.
AU - Plaisier, Peter W.
AU - Daniels, Johannes M. A.
AU - Thunnissen, Erik
AU - Radonic, Teodora
N1 - Funding Information: This study was supported by a grant of ORAS (Oncological Research Albert Schweitzer Hospital). Publisher Copyright: © 2023 The Authors
PY - 2023/12/1
Y1 - 2023/12/1
N2 - Primary diagnosis of bronchial carcinoids (BC) is always made on biopsies and additional immunohistochemistry (IHC) is often necessary. In the present study we investigated the concordance of common diagnostic (synaptophysin, chromogranin, CD56 and INSM-1) and potential prognostic (OTP, CD44, Rb and p16) IHC markers between the preoperative biopsies and resections of in total 64 BCs, 26 typical (41 %) and 38 atypical (59 %) carcinoid tumors. Synaptophysin and chromogranin had 100 % concordance in all resected carcinoids and paired diagnostic biopsies. Synaptophysin was not affected by variable expression in biopsies compared to chromogranin, CD56 and INSM-1. Notably, INSM-1 IHC was false negative in 8 % of biopsies. Of the novel and potential prognostic markers, only CD44 showed 100 % concordance between biopsies and resections, while OTP showed two (4 %) false negative results in paired biopsies. While Rb IHC was false negative in 8 % of biopsies, no strong and diffuse pattern of p16 expression was observed. In this study, most false negative IHC results (85 %, 22/26) were observed in small flexible biopsies. Taken together, our data suggest excellent concordance of synaptophysin and CD44 on the preoperative biopsy samples, while other neuroendocrine markers, Rb and OTP should be interpreted with caution, especially in small biopsies.
AB - Primary diagnosis of bronchial carcinoids (BC) is always made on biopsies and additional immunohistochemistry (IHC) is often necessary. In the present study we investigated the concordance of common diagnostic (synaptophysin, chromogranin, CD56 and INSM-1) and potential prognostic (OTP, CD44, Rb and p16) IHC markers between the preoperative biopsies and resections of in total 64 BCs, 26 typical (41 %) and 38 atypical (59 %) carcinoid tumors. Synaptophysin and chromogranin had 100 % concordance in all resected carcinoids and paired diagnostic biopsies. Synaptophysin was not affected by variable expression in biopsies compared to chromogranin, CD56 and INSM-1. Notably, INSM-1 IHC was false negative in 8 % of biopsies. Of the novel and potential prognostic markers, only CD44 showed 100 % concordance between biopsies and resections, while OTP showed two (4 %) false negative results in paired biopsies. While Rb IHC was false negative in 8 % of biopsies, no strong and diffuse pattern of p16 expression was observed. In this study, most false negative IHC results (85 %, 22/26) were observed in small flexible biopsies. Taken together, our data suggest excellent concordance of synaptophysin and CD44 on the preoperative biopsy samples, while other neuroendocrine markers, Rb and OTP should be interpreted with caution, especially in small biopsies.
KW - Bronchial carcinoid
KW - CD44
KW - INSM-1
KW - Immunohistochemistry
KW - OTP
KW - Synaptophysin
UR - http://www.scopus.com/inward/record.url?scp=85168347635&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.anndiagpath.2023.152181
DO - https://doi.org/10.1016/j.anndiagpath.2023.152181
M3 - Article
C2 - 37598464
SN - 1092-9134
VL - 67
JO - Annals of Diagnostic Pathology
JF - Annals of Diagnostic Pathology
M1 - 152181
ER -