In silicio search for genes encoding peroxisomal proteins in Saccharomyces cerevisiae

A. J. Kal; E. H. Hettema; M. van den Berg; M. G. Koerkamp; L. van Ijlst; B. Distel; H. F. Tabak

doi:https://doi.org/10.1385/CBB:32:1-3:01

In silicio search for genes encoding peroxisomal proteins in Saccharomyces cerevisiae

A. J. Kal, E. H. Hettema, M. van den Berg, M. G. Koerkamp, L. van Ijlst, B. Distel, H. F. Tabak

Other Departments

Research output: Contribution to journal › Article › Academic › peer-review

13 Citations (Scopus)

Abstract

The biogenesis of peroxisomes involves the synthesis of new proteins that after, completion of translation, are targeted to the organelle by virtue of peroxisomal targeting signals (PTS). Two types of PTSs have been well characterized for import of matrix proteins (PTS1 and PTS2). Induction of the genes encoding these matrix proteins takes place in oleate-containing medium and is mediated via an oleate response element (ORE) present in the region preceding these genes. The authors have searched the yeast genome for OREs preceding open reading frames (ORFs), and for ORFs that contain either a PTS1 or PTS2. Of the ORFs containing an ORE, as well as either a PTS1 or a PTS2, many were known to encode bona fide peroxisomal matrix proteins. In addition, candidate genes were identified as encoding putative new peroxisomal proteins. For one case, subcellular location studies validated the in silicio prediction. This gene encodes a new peroxisomal thioesterase

Original language	English
Pages (from-to)	1-8
Journal	Cell Biochemistry and Biophysics
Volume	32 Spring
DOIs	https://doi.org/10.1385/CBB:32:1-3:01
Publication status	Published - 2000

Access to Document

https://doi.org/10.1385/CBB:32:1-3:01

Cite this

@article{a2698da202284154975e1f3b81a99215,

title = "In silicio search for genes encoding peroxisomal proteins in Saccharomyces cerevisiae",

abstract = "The biogenesis of peroxisomes involves the synthesis of new proteins that after, completion of translation, are targeted to the organelle by virtue of peroxisomal targeting signals (PTS). Two types of PTSs have been well characterized for import of matrix proteins (PTS1 and PTS2). Induction of the genes encoding these matrix proteins takes place in oleate-containing medium and is mediated via an oleate response element (ORE) present in the region preceding these genes. The authors have searched the yeast genome for OREs preceding open reading frames (ORFs), and for ORFs that contain either a PTS1 or PTS2. Of the ORFs containing an ORE, as well as either a PTS1 or a PTS2, many were known to encode bona fide peroxisomal matrix proteins. In addition, candidate genes were identified as encoding putative new peroxisomal proteins. For one case, subcellular location studies validated the in silicio prediction. This gene encodes a new peroxisomal thioesterase",

author = "Kal, {A. J.} and Hettema, {E. H.} and {van den Berg}, M. and Koerkamp, {M. G.} and {van Ijlst}, L. and B. Distel and Tabak, {H. F.}",

year = "2000",

doi = "https://doi.org/10.1385/CBB:32:1-3:01",

language = "English",

volume = "32 Spring",

pages = "1--8",

journal = "Cell Biochemistry and Biophysics",

issn = "1085-9195",

publisher = "Humana Press",

}

TY - JOUR

T1 - In silicio search for genes encoding peroxisomal proteins in Saccharomyces cerevisiae

AU - Kal, A. J.

AU - Hettema, E. H.

AU - van den Berg, M.

AU - Koerkamp, M. G.

AU - van Ijlst, L.

AU - Distel, B.

AU - Tabak, H. F.

PY - 2000

Y1 - 2000

N2 - The biogenesis of peroxisomes involves the synthesis of new proteins that after, completion of translation, are targeted to the organelle by virtue of peroxisomal targeting signals (PTS). Two types of PTSs have been well characterized for import of matrix proteins (PTS1 and PTS2). Induction of the genes encoding these matrix proteins takes place in oleate-containing medium and is mediated via an oleate response element (ORE) present in the region preceding these genes. The authors have searched the yeast genome for OREs preceding open reading frames (ORFs), and for ORFs that contain either a PTS1 or PTS2. Of the ORFs containing an ORE, as well as either a PTS1 or a PTS2, many were known to encode bona fide peroxisomal matrix proteins. In addition, candidate genes were identified as encoding putative new peroxisomal proteins. For one case, subcellular location studies validated the in silicio prediction. This gene encodes a new peroxisomal thioesterase

AB - The biogenesis of peroxisomes involves the synthesis of new proteins that after, completion of translation, are targeted to the organelle by virtue of peroxisomal targeting signals (PTS). Two types of PTSs have been well characterized for import of matrix proteins (PTS1 and PTS2). Induction of the genes encoding these matrix proteins takes place in oleate-containing medium and is mediated via an oleate response element (ORE) present in the region preceding these genes. The authors have searched the yeast genome for OREs preceding open reading frames (ORFs), and for ORFs that contain either a PTS1 or PTS2. Of the ORFs containing an ORE, as well as either a PTS1 or a PTS2, many were known to encode bona fide peroxisomal matrix proteins. In addition, candidate genes were identified as encoding putative new peroxisomal proteins. For one case, subcellular location studies validated the in silicio prediction. This gene encodes a new peroxisomal thioesterase

U2 - https://doi.org/10.1385/CBB:32:1-3:01

DO - https://doi.org/10.1385/CBB:32:1-3:01

M3 - Article

C2 - 11330035

SN - 1085-9195

VL - 32 Spring

SP - 1

EP - 8

JO - Cell Biochemistry and Biophysics

JF - Cell Biochemistry and Biophysics

ER -