In vitro toxicity studies of polymer-coated gold nanorods

Raja G. Rayavarapu; Wilma Petersen; Liesbeth Hartsuiker; Patrick Chin; Hans Janssen; Fijs W. B. van Leeuwen; Cees Otto; Srirang Manohar; Ton G. van Leeuwen

doi:https://doi.org/10.1088/0957-4484/21/14/145101

In vitro toxicity studies of polymer-coated gold nanorods

Raja G. Rayavarapu, Wilma Petersen, Liesbeth Hartsuiker, Patrick Chin, Hans Janssen, Fijs W. B. van Leeuwen, Cees Otto, Srirang Manohar, Ton G. van Leeuwen

Research output: Contribution to journal › Article › Academic › peer-review

134 Citations (Scopus)

Abstract

We evaluated cellular responses to polymer-treated gold nanorods, which were synthesized using the standard wet-chemistry method that utilizes hexadecyltrimethylammonium bromide (CTAB). The nanorod dispersions were coated with either polystyrene sulfonate (PSS) or polyethylene glycol (PEG). Two sizes of nanorods were tested, with optical responses peaking at 628 and 773 nm. The cells were from mammary adenocarcinoma (SKBR3), Chinese Hamster Ovary (CHO), mouse myoblast (C2C12) and Human Leukemia (HL60) cell lines. Their mitochondrial function following exposure to the nanorods were assessed using the MTS assay. We found PEGylated particles to have superior biocompatibility compared with PSS-coated nanorods, which showed substantial cytotoxicity. Electron microscopy showed no cellular uptake of PEGylated particles compared with their PSS counterparts. PEGylated gold nanorods also exhibited better dispersion stability in the presence of cell growth medium; PSS-coated rods tended to flocculate or cluster. In the case of the PSS particles, toxicity correlated with surface area across the two sizes of nanorods studied

Original language	English
Pages (from-to)	145101
Journal	Nanotechnology
Volume	21
Issue number	14
DOIs	https://doi.org/10.1088/0957-4484/21/14/145101
Publication status	Published - 2010

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https://doi.org/10.1088/0957-4484/21/14/145101

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@article{00cb3cb03eb345de994224b37e205794,

title = "In vitro toxicity studies of polymer-coated gold nanorods",

abstract = "We evaluated cellular responses to polymer-treated gold nanorods, which were synthesized using the standard wet-chemistry method that utilizes hexadecyltrimethylammonium bromide (CTAB). The nanorod dispersions were coated with either polystyrene sulfonate (PSS) or polyethylene glycol (PEG). Two sizes of nanorods were tested, with optical responses peaking at 628 and 773 nm. The cells were from mammary adenocarcinoma (SKBR3), Chinese Hamster Ovary (CHO), mouse myoblast (C2C12) and Human Leukemia (HL60) cell lines. Their mitochondrial function following exposure to the nanorods were assessed using the MTS assay. We found PEGylated particles to have superior biocompatibility compared with PSS-coated nanorods, which showed substantial cytotoxicity. Electron microscopy showed no cellular uptake of PEGylated particles compared with their PSS counterparts. PEGylated gold nanorods also exhibited better dispersion stability in the presence of cell growth medium; PSS-coated rods tended to flocculate or cluster. In the case of the PSS particles, toxicity correlated with surface area across the two sizes of nanorods studied",

author = "Rayavarapu, {Raja G.} and Wilma Petersen and Liesbeth Hartsuiker and Patrick Chin and Hans Janssen and {van Leeuwen}, {Fijs W. B.} and Cees Otto and Srirang Manohar and {van Leeuwen}, {Ton G.}",

year = "2010",

doi = "https://doi.org/10.1088/0957-4484/21/14/145101",

language = "English",

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journal = "Nanotechnology",

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T1 - In vitro toxicity studies of polymer-coated gold nanorods

AU - Rayavarapu, Raja G.

AU - Petersen, Wilma

AU - Hartsuiker, Liesbeth

AU - Chin, Patrick

AU - Janssen, Hans

AU - van Leeuwen, Fijs W. B.

AU - Otto, Cees

AU - Manohar, Srirang

AU - van Leeuwen, Ton G.

PY - 2010

Y1 - 2010

N2 - We evaluated cellular responses to polymer-treated gold nanorods, which were synthesized using the standard wet-chemistry method that utilizes hexadecyltrimethylammonium bromide (CTAB). The nanorod dispersions were coated with either polystyrene sulfonate (PSS) or polyethylene glycol (PEG). Two sizes of nanorods were tested, with optical responses peaking at 628 and 773 nm. The cells were from mammary adenocarcinoma (SKBR3), Chinese Hamster Ovary (CHO), mouse myoblast (C2C12) and Human Leukemia (HL60) cell lines. Their mitochondrial function following exposure to the nanorods were assessed using the MTS assay. We found PEGylated particles to have superior biocompatibility compared with PSS-coated nanorods, which showed substantial cytotoxicity. Electron microscopy showed no cellular uptake of PEGylated particles compared with their PSS counterparts. PEGylated gold nanorods also exhibited better dispersion stability in the presence of cell growth medium; PSS-coated rods tended to flocculate or cluster. In the case of the PSS particles, toxicity correlated with surface area across the two sizes of nanorods studied

AB - We evaluated cellular responses to polymer-treated gold nanorods, which were synthesized using the standard wet-chemistry method that utilizes hexadecyltrimethylammonium bromide (CTAB). The nanorod dispersions were coated with either polystyrene sulfonate (PSS) or polyethylene glycol (PEG). Two sizes of nanorods were tested, with optical responses peaking at 628 and 773 nm. The cells were from mammary adenocarcinoma (SKBR3), Chinese Hamster Ovary (CHO), mouse myoblast (C2C12) and Human Leukemia (HL60) cell lines. Their mitochondrial function following exposure to the nanorods were assessed using the MTS assay. We found PEGylated particles to have superior biocompatibility compared with PSS-coated nanorods, which showed substantial cytotoxicity. Electron microscopy showed no cellular uptake of PEGylated particles compared with their PSS counterparts. PEGylated gold nanorods also exhibited better dispersion stability in the presence of cell growth medium; PSS-coated rods tended to flocculate or cluster. In the case of the PSS particles, toxicity correlated with surface area across the two sizes of nanorods studied

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DO - https://doi.org/10.1088/0957-4484/21/14/145101

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SN - 0957-4484

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JO - Nanotechnology

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