TY - JOUR
T1 - Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source
AU - Boonkaew, Bootsakorn
AU - Tapeng, Lunnathaya
AU - Netsrithong, Ratchapong
AU - Vatanashevanopakorn, Chinnavuth
AU - Pattanapanyasat, Kovit
AU - Wattanapanitch, Methichit
N1 - Funding Information: This study was supported by Siriraj Research Fund, Faculty of Medicine Siriraj Hospital, Mahidol University, Grant number (IO) R016033017 and the Thailand Research Fund (TRF) – Distinguished Research Professor Grant, contract no. DPG5980001 and IRG 5980006. MW and CV are supported by Chalermphrakiat Grant, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand. Funding Information: This study was supported by Siriraj Research Fund, Faculty of Medicine Siriraj Hospital , Mahidol University , Grant number (IO) R016033017 and the Thailand Research Fund (TRF) – Distinguished Research Professor Grant, contract no. DPG5980001 and IRG 5980006 . MW and CV are supported by Chalermphrakiat Grant, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand. Publisher Copyright: © 2018 The Authors
PY - 2018/8
Y1 - 2018/8
N2 - In this study, we used hair follicle keratinocytes for reprogramming. Collection of plucked hairs offers advantages over other somatic cells because no medical professional or operation room is required. Keratinocytes were isolated from plucked hairs of a 21-year-old healthy woman and characterized for the expression of cytokeratin 14 (CK14). Reprogramming of keratinocytes was performed using Sendai virus. Further characterization of the keratinocyte-derived iPSC line (designated as MUSIi006-A) confirmed that the cell line was pluripotent, free from Sendai viral genome and transgenes, and retained normal karyotype. Our method represents an easy, non-invasive and efficient approach for iPSC generation from hair samples.
AB - In this study, we used hair follicle keratinocytes for reprogramming. Collection of plucked hairs offers advantages over other somatic cells because no medical professional or operation room is required. Keratinocytes were isolated from plucked hairs of a 21-year-old healthy woman and characterized for the expression of cytokeratin 14 (CK14). Reprogramming of keratinocytes was performed using Sendai virus. Further characterization of the keratinocyte-derived iPSC line (designated as MUSIi006-A) confirmed that the cell line was pluripotent, free from Sendai viral genome and transgenes, and retained normal karyotype. Our method represents an easy, non-invasive and efficient approach for iPSC generation from hair samples.
UR - http://www.scopus.com/inward/record.url?scp=85050001250&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.scr.2018.07.007
DO - https://doi.org/10.1016/j.scr.2018.07.007
M3 - Article
C2 - 30031234
SN - 1873-5061
VL - 31
SP - 79
EP - 82
JO - Stem Cell Research
JF - Stem Cell Research
ER -