Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

A. E. Saarnak; T. Rodrigues; J. Schwartz; A. L. Moore; T. A. Moore; D. Gust; M. J. C. van Gemert; H. J. C. M. Sterenborg; S. Thomsen

doi:https://doi.org/10.1007/BF00592957

Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

A. E. Saarnak, T. Rodrigues, J. Schwartz, A. L. Moore, T. A. Moore, D. Gust, M. J. C. van Gemert, H. J. C. M. Sterenborg, S. Thomsen

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Abstract

We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)(3), mas injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm(2) of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin. covered and uncovered tumour, The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour, We used a one-dimensional layered tissue model to analyse the data, In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity

Original language	English
Pages (from-to)	22-31
Journal	Lasers in Medical Science
Volume	13
Issue number	1
DOIs	https://doi.org/10.1007/BF00592957
Publication status	Published - 1998

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https://doi.org/10.1007/BF00592957

Cite this

@article{0b8e048af33848848f97d239b67c6c1e,

title = "Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue",

abstract = "We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)(3), mas injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm(2) of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin. covered and uncovered tumour, The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour, We used a one-dimensional layered tissue model to analyse the data, In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity",

author = "Saarnak, {A. E.} and T. Rodrigues and J. Schwartz and Moore, {A. L.} and Moore, {T. A.} and D. Gust and {van Gemert}, {M. J. C.} and Sterenborg, {H. J. C. M.} and S. Thomsen",

year = "1998",

doi = "https://doi.org/10.1007/BF00592957",

language = "English",

volume = "13",

pages = "22--31",

journal = "Lasers in Medical Science",

issn = "0268-8921",

publisher = "Springer London",

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TY - JOUR

T1 - Influence of tumour depth, blood absorption and autofluorescence on measurements of exogenous fluorophores in tissue

AU - Saarnak, A. E.

AU - Rodrigues, T.

AU - Schwartz, J.

AU - Moore, A. L.

AU - Moore, T. A.

AU - Gust, D.

AU - van Gemert, M. J. C.

AU - Sterenborg, H. J. C. M.

AU - Thomsen, S.

PY - 1998

Y1 - 1998

N2 - We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)(3), mas injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm(2) of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin. covered and uncovered tumour, The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour, We used a one-dimensional layered tissue model to analyse the data, In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity

AB - We investigated the influence of tumour depth and differences in blood concentration and autofluorescence between tumour and normal tissue on the fluorescence of a tumour-localising agent. Carotenoporphyrin, CP(Me)(3), mas injected into rats and nude mice with intradermal tumours. On the tumours an incision was made, uncovering 2 mm(2) of the tumour, and fluorescence measurements, including excitation-emission maps and fluorescence ratios, were made on skin. covered and uncovered tumour, The measured fluorescence ratio in the uncovered tumour showed a three- to tenfold increase compared to the covered tumour, We used a one-dimensional layered tissue model to analyse the data, In conclusion, even with a high tumour-selectivity deeper lying tumours cannot always be detected, particularly if the tumour has a high blood concentration or low autofluorescence intensity

U2 - https://doi.org/10.1007/BF00592957

DO - https://doi.org/10.1007/BF00592957

M3 - Article

SN - 0268-8921

VL - 13

SP - 22

EP - 31

JO - Lasers in Medical Science

JF - Lasers in Medical Science

IS - 1

ER -