Initiation of replication of the Escherichia coli chromosomal origin reconstituted with purified enzymes

J. M. Kaguni; L. L. Bertsch; D. Bramhill; J. E. Flynn; R. S. Fuller; B. Funnell; S. Maki; T. Ogawa; K. Ogawa; A. van der Ende

Initiation of replication of the Escherichia coli chromosomal origin reconstituted with purified enzymes

J. M. Kaguni, L. L. Bertsch, D. Bramhill, J. E. Flynn, R. S. Fuller, B. Funnell, S. Maki, T. Ogawa, K. Ogawa, A. van der Ende

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Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

A mixture of purified proteins has replaced a crude enzyme fraction capable of efficient replication of oriC-containing plasmids. The reconstituted enzyme system contains proteins which provide initiation, replication, and specificity functions required for dnaA-dependent replication specific for an oriC template. Replication can be separated into successive stages of RNA synthesis and DNA replication. Isolation of an intermediate no longer requiring RNA polymerase action requires the presence of dnaA protein, DNA gyrase, dnaB protein and dnaC protein. Intermediate formation likely involves binding of dnaA protein to a 9-bp sequence present 4 times as inverted repeats within the chromosomal origin sequence

Original language	English
Pages (from-to)	141-150
Journal	Basic life sciences
Volume	30
Publication status	Published - 1985

Cite this

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title = "Initiation of replication of the Escherichia coli chromosomal origin reconstituted with purified enzymes",

abstract = "A mixture of purified proteins has replaced a crude enzyme fraction capable of efficient replication of oriC-containing plasmids. The reconstituted enzyme system contains proteins which provide initiation, replication, and specificity functions required for dnaA-dependent replication specific for an oriC template. Replication can be separated into successive stages of RNA synthesis and DNA replication. Isolation of an intermediate no longer requiring RNA polymerase action requires the presence of dnaA protein, DNA gyrase, dnaB protein and dnaC protein. Intermediate formation likely involves binding of dnaA protein to a 9-bp sequence present 4 times as inverted repeats within the chromosomal origin sequence",

author = "Kaguni, {J. M.} and Bertsch, {L. L.} and D. Bramhill and Flynn, {J. E.} and Fuller, {R. S.} and B. Funnell and S. Maki and T. Ogawa and K. Ogawa and {van der Ende}, A.",

year = "1985",

language = "English",

volume = "30",

pages = "141--150",

journal = "Basic life sciences",

issn = "0090-5542",

publisher = "Springer GmbH & Co, Auslieferungs-Gesellschaf",

}

TY - JOUR

T1 - Initiation of replication of the Escherichia coli chromosomal origin reconstituted with purified enzymes

AU - Kaguni, J. M.

AU - Bertsch, L. L.

AU - Bramhill, D.

AU - Flynn, J. E.

AU - Fuller, R. S.

AU - Funnell, B.

AU - Maki, S.

AU - Ogawa, T.

AU - Ogawa, K.

AU - van der Ende, A.

PY - 1985

Y1 - 1985

N2 - A mixture of purified proteins has replaced a crude enzyme fraction capable of efficient replication of oriC-containing plasmids. The reconstituted enzyme system contains proteins which provide initiation, replication, and specificity functions required for dnaA-dependent replication specific for an oriC template. Replication can be separated into successive stages of RNA synthesis and DNA replication. Isolation of an intermediate no longer requiring RNA polymerase action requires the presence of dnaA protein, DNA gyrase, dnaB protein and dnaC protein. Intermediate formation likely involves binding of dnaA protein to a 9-bp sequence present 4 times as inverted repeats within the chromosomal origin sequence

AB - A mixture of purified proteins has replaced a crude enzyme fraction capable of efficient replication of oriC-containing plasmids. The reconstituted enzyme system contains proteins which provide initiation, replication, and specificity functions required for dnaA-dependent replication specific for an oriC template. Replication can be separated into successive stages of RNA synthesis and DNA replication. Isolation of an intermediate no longer requiring RNA polymerase action requires the presence of dnaA protein, DNA gyrase, dnaB protein and dnaC protein. Intermediate formation likely involves binding of dnaA protein to a 9-bp sequence present 4 times as inverted repeats within the chromosomal origin sequence

M3 - Article

C2 - 2990405

SN - 0090-5542

VL - 30

SP - 141

EP - 150

JO - Basic life sciences

JF - Basic life sciences

ER -