TY - JOUR
T1 - Interactions between colon cancer cells and hepatocytes in rats in relation to metastasis
AU - Mook, O. R. F.
AU - van Marle, J.
AU - Jonges, R.
AU - Vreeling-Sindelárová, H.
AU - Frederiks, W. M.
AU - van Noorden, C. J. F.
PY - 2008
Y1 - 2008
N2 - Adhesion of cancer cells to endothelium is considered an essential step in metastasis. However, we have shown in a previous study that when rat colon cancer cells are administered to the vena portae, they get stuck mechanically in liver sinusoids. Then, endothelial cells retract rapidly and cancer cells bind to hepatocytes. We investigated the molecular nature of these interactions between colon cancer cells and hepatocytes. Cancer cells in coculture with hepatocytes became rapidly activated with distinct morphological changes. Cancer cells formed long cytoplasmic protrusions towards hepatocytes in their close vicinity and these protrusions attached to microvilli of hepatocytes. Then, adhering membrane areas were formed by both cell types. Integrin subunits alpha v, alpha 6 and beta 1 but not alpha L, beta 2, beta 3 and CD44 and CD44v6 were expressed on the cancer cells. In conclusion, colon cancer cells show an active behaviour to bind to hepatocytes, likely involving the integrin subunits alpha v, alpha 6 and beta 1, indicating that early events in colon cancer metastasis in liver are distinctly different than assumed thus far
AB - Adhesion of cancer cells to endothelium is considered an essential step in metastasis. However, we have shown in a previous study that when rat colon cancer cells are administered to the vena portae, they get stuck mechanically in liver sinusoids. Then, endothelial cells retract rapidly and cancer cells bind to hepatocytes. We investigated the molecular nature of these interactions between colon cancer cells and hepatocytes. Cancer cells in coculture with hepatocytes became rapidly activated with distinct morphological changes. Cancer cells formed long cytoplasmic protrusions towards hepatocytes in their close vicinity and these protrusions attached to microvilli of hepatocytes. Then, adhering membrane areas were formed by both cell types. Integrin subunits alpha v, alpha 6 and beta 1 but not alpha L, beta 2, beta 3 and CD44 and CD44v6 were expressed on the cancer cells. In conclusion, colon cancer cells show an active behaviour to bind to hepatocytes, likely involving the integrin subunits alpha v, alpha 6 and beta 1, indicating that early events in colon cancer metastasis in liver are distinctly different than assumed thus far
U2 - https://doi.org/10.1111/j.1582-4934.2008.00242.x
DO - https://doi.org/10.1111/j.1582-4934.2008.00242.x
M3 - Article
C2 - 18208563
SN - 1582-1838
VL - 12
SP - 2052
EP - 2061
JO - Journal of cellular and molecular medicine
JF - Journal of cellular and molecular medicine
IS - 5B
ER -