Abstract
Recirculating lymphocytes specifically adhere to and migrate through endothelium lining high endothelial venules (HEV), using specific and non-specific receptor-ligand systems. Interferons (IFN) profoundly affect the traffic of lymphocytes. Therefore the effects of interferon-gamma (IFN-gamma) on lymphocytes with respect to their interaction with HEV endothelium in lymph nodes were studied using the frozen section assay. In addition the organ-specificity of T- and B-lymphocyte adherence to HEV was investigated. Lymphocytes from spleen and peripheral lymph nodes showed increased binding to HEV of up to 35% compared to control lymphocytes after in vivo administration of IFN-gamma. The specificity of their binding did not change in terms of T:B-cell ratios. Increased adherence was also found by preincubation of lymphocytes with IFN-gamma in The enhanced binding was not due to increased expression of Mel-14 homing receptors and LFA-1 molecules on T and B lymphocytes, as shown by fluorescence-activated cell sorter analysis and antibody-blocking studies in the frozen section assay. Apparently IFN-gamma induces an additional Mel-14-independent and LFA-1-independent adhesion mechanism on lymphocytes.
Original language | English |
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Pages (from-to) | 221-6 |
Number of pages | 6 |
Journal | Immunology |
Volume | 68 |
Issue number | 2 |
Publication status | Published - Oct 1989 |
Keywords
- Animals
- B-Lymphocytes/drug effects
- Cell Adhesion/drug effects
- Endothelium, Lymphatic/physiology
- Endothelium/physiology
- Interferon-gamma/pharmacology
- Male
- Rats
- Rats, Inbred Strains
- Recombinant Proteins
- T-Lymphocytes/drug effects