TY - JOUR
T1 - Intragraft FOXP3 Protein or mRNA During Acute Renal Allograft Rejection Correlates With Inflammation, Fibrosis, and Poor Renal Outcome
AU - Yapici, Unsal
AU - Bemelman, Fréderike J.
AU - Scheepstra, Cornelis G.
AU - Roelofs, Joris J. T. H.
AU - Claessen, Nike
AU - van der Loos, Chris
AU - van Donselaar-van der Pant, Karlijn
AU - Bouts, Antonia H. M.
AU - Idu, Mirza M.
AU - Rowshani, Ajda T.
AU - ten Berge, Ineke J. M.
AU - Florquin, Sandrine
PY - 2009
Y1 - 2009
N2 - Background: Forkhead box (FOXP3) is considered to be a specific marker for regulatory T cells. The aim of this study was to correlate intragraft FOXP3 at mRNA and cellular levels during renal allograft rejection to response to therapy and late clinical outcome. Methods: Immunostainings and quantitative reverse-transcriptase polymerase chain reaction for FOXP3, CD3, and transforming growth factor (TGF)-beta were performed and results were related to histopathologic and clinical outcome. Results: A good correlation between immunohistochemical analysis and mRNA levels for both CD3 and FOXP3 was observed. Intragraft FOYP3 was significantly related to tubulitis and interstitial fibrosis. A strong correlation was found between FOXP3 and CD3 mRNA and between FOXP3 and TGF-beta mRNA. No correlation was found between FOXP3 and response to therapy. Discussion: In conclusion, intragraft FOXP3 at both cellular and molecular levels parallels T-cell infiltration during acute rejection. FOXP3 does not predict response to antirejection therapy. FOXP3 correlates with renal fibrosis, TGF-beta, and poor late renal outcome
AB - Background: Forkhead box (FOXP3) is considered to be a specific marker for regulatory T cells. The aim of this study was to correlate intragraft FOXP3 at mRNA and cellular levels during renal allograft rejection to response to therapy and late clinical outcome. Methods: Immunostainings and quantitative reverse-transcriptase polymerase chain reaction for FOXP3, CD3, and transforming growth factor (TGF)-beta were performed and results were related to histopathologic and clinical outcome. Results: A good correlation between immunohistochemical analysis and mRNA levels for both CD3 and FOXP3 was observed. Intragraft FOYP3 was significantly related to tubulitis and interstitial fibrosis. A strong correlation was found between FOXP3 and CD3 mRNA and between FOXP3 and TGF-beta mRNA. No correlation was found between FOXP3 and response to therapy. Discussion: In conclusion, intragraft FOXP3 at both cellular and molecular levels parallels T-cell infiltration during acute rejection. FOXP3 does not predict response to antirejection therapy. FOXP3 correlates with renal fibrosis, TGF-beta, and poor late renal outcome
U2 - https://doi.org/10.1097/TP.0b013e3181a24a4b
DO - https://doi.org/10.1097/TP.0b013e3181a24a4b
M3 - Article
C2 - 19424039
SN - 0041-1337
VL - 87
SP - 1377
EP - 1380
JO - Transplantation
JF - Transplantation
IS - 9
ER -