TY - JOUR
T1 - Intragraft Tubular Vimentin and CD44 Expression Correlate With Long-Term Renal Allograft Function and Interstitial Fibrosis and Tubular Atrophy
AU - Kers, Jesper
AU - Xu-Dubois, Yi-Chun
AU - Rondeau, Eric
AU - Claessen, Nike
AU - Idu, Mirza M.
AU - Roelofs, Joris J. T. H.
AU - Bemelman, Fréderike J.
AU - ten Berge, Ineke J. M.
AU - Florquin, Sandrine
PY - 2010
Y1 - 2010
N2 - Background. Development of interstitial fibrosis and tubular atrophy (IF/TA) is the main histologic feature involved in renal allograft deterioration. The aim of this study was to validate whether de novo tubular expression of CD44 (transmembrane glycoprotein) and vimentin (mesenchymal cell marker), both involved in renal fibrosis, can operate as surrogate markers for late IF/TA and renal function. Furthermore, we wanted to establish the interrater reproducibility for the scoring system, which can be a problem in histologic assessments. Methods. Six-month protocol renal allograft biopsies (n = 30 for matching 12 months estimated glomerular filtration rate (eGFR) from which 20 matched the 12-month protocol biopsy) were immunostained for CD44 and vimentin, semiquantitatively scored by three observers of two centers, and correlated with IF/TA and eGFR at 12 months. Results. The interobserver agreement was excellent for CD44 (Kendall's W-coefficient: 0.69; P <0.001) and vimentin (Kendall's W-coefficient: 0.79; P <0.001). CD44 and vimentin expression at 6 months were significantly correlated with IF/TA (rho = 0.481 for CD44 and rho = 0.619 for vimentin) and eGFR (rho = -0.569 for CD44 and rho = -0.376 for vimentin) at 12 months. Keywords: Kidney transplantation, Biomarkers, Chronic allograft dysfunction, CD44, Vimentin, Epithelial to mesenchymal transition
AB - Background. Development of interstitial fibrosis and tubular atrophy (IF/TA) is the main histologic feature involved in renal allograft deterioration. The aim of this study was to validate whether de novo tubular expression of CD44 (transmembrane glycoprotein) and vimentin (mesenchymal cell marker), both involved in renal fibrosis, can operate as surrogate markers for late IF/TA and renal function. Furthermore, we wanted to establish the interrater reproducibility for the scoring system, which can be a problem in histologic assessments. Methods. Six-month protocol renal allograft biopsies (n = 30 for matching 12 months estimated glomerular filtration rate (eGFR) from which 20 matched the 12-month protocol biopsy) were immunostained for CD44 and vimentin, semiquantitatively scored by three observers of two centers, and correlated with IF/TA and eGFR at 12 months. Results. The interobserver agreement was excellent for CD44 (Kendall's W-coefficient: 0.69; P <0.001) and vimentin (Kendall's W-coefficient: 0.79; P <0.001). CD44 and vimentin expression at 6 months were significantly correlated with IF/TA (rho = 0.481 for CD44 and rho = 0.619 for vimentin) and eGFR (rho = -0.569 for CD44 and rho = -0.376 for vimentin) at 12 months. Keywords: Kidney transplantation, Biomarkers, Chronic allograft dysfunction, CD44, Vimentin, Epithelial to mesenchymal transition
U2 - https://doi.org/10.1097/TP.0b013e3181e86b42
DO - https://doi.org/10.1097/TP.0b013e3181e86b42
M3 - Article
C2 - 20588206
SN - 0041-1337
VL - 90
SP - 502
EP - 509
JO - Transplantation
JF - Transplantation
IS - 5
ER -