TY - JOUR
T1 - Involvement of Ser103 of the Rh polypeptides in G epitope formation
AU - Faas, B. H.
AU - Beckers, E. A.
AU - Simsek, S.
AU - Overbeeke, M. A.
AU - Pepper, R.
AU - van Rhenen, D. J.
AU - von dem Borne, A. E.
AU - van der Schoot, C. E.
PY - 1996
Y1 - 1996
N2 - Almost all red cells that carry D and/or C antigens also express the G antigen (Rh12). A study was conducted on the molecular background of the G epitope. Two unrelated donors with the rare ccDEe, G- phenotype and one donor with the ccEe, G+ phenotype were studied. Genomic DNA and cDNA of these donors were studied with polymerase chain reaction, Southern blot, and sequence analysis, with special focus on exon 2, because it is only in this exon that there are supposed to be similarities between RHD and the RHC allele, but not between RHD and the RHc allele. In both ccDEe, G- donors, a nucleotide substitution was found in exon 2 of RHD; T307 was replaced by C307, which predicted a Ser->Pro substitution at amino acid position 103 of the D polypeptide. The ccEe, G+ donor carried the complete exon 2 of RHD. Moreover, despite the absence of all known D epitopes, this donor also carried RHD characteristics in exons 1 to 3 and exon 9 and further downstream. Ser103, encoded by exon 2 of the RH genes, is involved in G epitope formation
AB - Almost all red cells that carry D and/or C antigens also express the G antigen (Rh12). A study was conducted on the molecular background of the G epitope. Two unrelated donors with the rare ccDEe, G- phenotype and one donor with the ccEe, G+ phenotype were studied. Genomic DNA and cDNA of these donors were studied with polymerase chain reaction, Southern blot, and sequence analysis, with special focus on exon 2, because it is only in this exon that there are supposed to be similarities between RHD and the RHC allele, but not between RHD and the RHc allele. In both ccDEe, G- donors, a nucleotide substitution was found in exon 2 of RHD; T307 was replaced by C307, which predicted a Ser->Pro substitution at amino acid position 103 of the D polypeptide. The ccEe, G+ donor carried the complete exon 2 of RHD. Moreover, despite the absence of all known D epitopes, this donor also carried RHD characteristics in exons 1 to 3 and exon 9 and further downstream. Ser103, encoded by exon 2 of the RH genes, is involved in G epitope formation
U2 - https://doi.org/10.1046/j.1537-2995.1996.36696269508.x
DO - https://doi.org/10.1046/j.1537-2995.1996.36696269508.x
M3 - Article
C2 - 8669081
SN - 0041-1132
VL - 36
SP - 506
EP - 511
JO - Transfusion
JF - Transfusion
IS - 6
ER -