TY - JOUR
T1 - Isolation of gonadotrops from the pituitary of the African catfish, Clarias lazera - Morphological and physiological characterization of the purified cells
AU - de Leeuw, R.
AU - Goos, H. J.Th
AU - Peute, J.
AU - van Pelt, A. M.M.
AU - Burzawa-Gérard, E.
AU - van Oordt, P. G.W.J.
PY - 1984/6
Y1 - 1984/6
N2 - Dispersed pituitary cells from male African catfish, Clarias lazera, were fractionated in a density gradient of Percoll. Five fractions were isolated, consisting of about 6, 19, 39, 95 and 83% gonadotrops, respectively. The gonadotrops were identified by their ultrastructural characteristics, by immunocytochemistry, and by measuring their hormone content. After one day in culture, in each fraction the secretion of gonadotropin could be stimulated by a luteinizing hormone-releasing hormone analogue, indicating that the cells had retained their functional integrity. Since the regulatory mechanisms of different cell types from the pituitary have some similarity, purification of the gonadotrops provides a model to study the regulation of gonadotropin secretion.
AB - Dispersed pituitary cells from male African catfish, Clarias lazera, were fractionated in a density gradient of Percoll. Five fractions were isolated, consisting of about 6, 19, 39, 95 and 83% gonadotrops, respectively. The gonadotrops were identified by their ultrastructural characteristics, by immunocytochemistry, and by measuring their hormone content. After one day in culture, in each fraction the secretion of gonadotropin could be stimulated by a luteinizing hormone-releasing hormone analogue, indicating that the cells had retained their functional integrity. Since the regulatory mechanisms of different cell types from the pituitary have some similarity, purification of the gonadotrops provides a model to study the regulation of gonadotropin secretion.
KW - African catfish (Clarias lazera)
KW - Cell culture
KW - Gonadotropin secretion
KW - Gonadotrops
KW - Isolation
UR - http://www.scopus.com/inward/record.url?scp=0021225092&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/BF00217237
DO - https://doi.org/10.1007/BF00217237
M3 - Article
C2 - 6380741
SN - 0302-766X
VL - 236
SP - 669
EP - 675
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 3
ER -