TY - CHAP
T1 - Isolation of pig bone marrow-derived mesenchymal stem cells
AU - Feyen, Dries A. M.
AU - van den Akker, Frederieke
AU - Noort, Willy
AU - Chamuleau, Steven A. J.
AU - Doevendans, Pieter A.
AU - Sluijter, Joost P. G.
PY - 2016
Y1 - 2016
N2 - Large animal models are an important preclinical tool for the evaluation of new interventions and their translation into clinical practice. The pig is a widely used animal model in multiple clinical fields, such as cardiology and orthopedics, and has been at the forefront of testing new therapeutics, including cell-based therapies. In the clinic, mesenchymal stem cells (MSCs) are used autologously, therefore isolated, and administrated into the same patient. For successful clinical translation of autologous approaches, the porcine model needs to test MSC in a similar manner. Since a limited number of MSCs can be isolated directly from the bone marrow, culturing techniques are needed to expand the population in vitro prior to therapeutic application. Here, we describe a protocol specifically tailored for the isolation and propagation of porcine-derived bone marrow MSCs.
AB - Large animal models are an important preclinical tool for the evaluation of new interventions and their translation into clinical practice. The pig is a widely used animal model in multiple clinical fields, such as cardiology and orthopedics, and has been at the forefront of testing new therapeutics, including cell-based therapies. In the clinic, mesenchymal stem cells (MSCs) are used autologously, therefore isolated, and administrated into the same patient. For successful clinical translation of autologous approaches, the porcine model needs to test MSC in a similar manner. Since a limited number of MSCs can be isolated directly from the bone marrow, culturing techniques are needed to expand the population in vitro prior to therapeutic application. Here, we describe a protocol specifically tailored for the isolation and propagation of porcine-derived bone marrow MSCs.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84990238827&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/27236674
U2 - https://doi.org/10.1007/978-1-4939-3584-0_12
DO - https://doi.org/10.1007/978-1-4939-3584-0_12
M3 - Chapter
C2 - 27236674
VL - 1416
T3 - Methods in Molecular Biology
SP - 225
EP - 232
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -