TY - JOUR
T1 - Label-free cell profiling
AU - Schasfoort, Richard B. M.
AU - Bentlage, Arthur E. H.
AU - Stojanovic, Ivan
AU - van der Kooi, Alex
AU - van der Schoot, Ellen
AU - Terstappen, Leon W. M. M.
AU - Vidarsson, Gestur
PY - 2013
Y1 - 2013
N2 - A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs
AB - A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs
U2 - https://doi.org/10.1016/j.ab.2013.04.001
DO - https://doi.org/10.1016/j.ab.2013.04.001
M3 - Article
C2 - 23583822
SN - 0003-2697
VL - 439
SP - 4
EP - 6
JO - Analytical biochemistry
JF - Analytical biochemistry
IS - 1
ER -