Abstract
Background: Activity of immunoglobulin (Ig)E-dependent histamine-releasing factor (HRF) is dependent on the IgE molecules bound to the surface of basophils. Sera capable of passively sensitizing basophils to release histamine to HRF were designated IgE(+) sera. IgE(+) and HRF have been suggested to play a role in late allergic reaction (LAR). Objective: The working hypothesis was tested that IgE(+) induces a LAR. Further, activity of HRF produced by mononuclear cells (HRFmn) was compared with that of recombinant HRF p23. Methods: Atopic patients (n = 82) were bronchially provoked with Dermatophagoides pteronyssinus extract and the change in forced expiratory volume in I second was monitored. A LAR was defined as forced expiratory volume in 1 second as percentage of baseline <80% 4 to 10 hours after allergen challenge. The presence of HRF-responsive IgE in serum was determined using basophils sensitized in vitro by serum. Results: The presence of HRFmn-responsive IgE (IgE(mn+)) in serum was shown not be essential for a LAR: 63% of the patients with a LAR had no IgE(mn+) in their serum. Further, 71% of patients with IgE(mn+) did not have a LAR. HRFmn and recombinant HRF p23 were not equivalent in the bioassay: serum of 38 of 82 atopic patients sensitized basophils to release histamine to HRFmn, whereas this was found with serum of 1 of 82 patients to HRF p23. Conclusions: The results do not support the hypothesis that IgE(mn+) induces a LAR, but do not exclude the alternative hypothesis that HRFs are released during a LAR and contribute to asthma severity
Original language | English |
---|---|
Pages (from-to) | 606-612 |
Journal | Annals of allergy, asthma & immunology |
Volume | 89 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2002 |